We narrowed to 424 results for: SUA

1. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   ...library of transformants for single colonies (this usually requires 10-20 plates) (see box below). This initial...

2. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   ...oligonucleotide, we aim to generate 50 to 100 mice and are usually successful in deriving mice carrying the intended...

3. Finding Your Perfect Job After University

   Type

   Blog Post

   ...not suit everyone. My career path may seem quite unusual but it has allowed me to try out working in many...

4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

   Type

   Blog Post

   ...to regulate gene expression (CRISPRi/CRISPRa), visualize specific genomic loci (CRISPR-imaging), or to ...

5. A History of Genome Engineering in Popular Culture

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   Blog Post

   ...  Genetic Engineering Era: Pre-CRISPR  Having visualized and understood the DNA, the next milestone for...

6. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

   Type

   Blog Post

   ...to cell-type specific promoters; however, those usually drive weak expression of downstream genes. Strong...

7. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   ...differences. The original creators of this technique visualized the ligation product in a gel, but we can now...

8. Transferable Skills Guide: Teamwork

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   Blog Post

   ...experiments I’d been learning about for years were usually the products of teams: Watson, Crick, & Franklin...

9. Quick Guide to All Things Lentivirus

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   Blog Post

   ... will first need to transfect producing cells, usually 293T cells, with your transfer plasmid and your...

10. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post

    ... digestion products (which could be easier to visualize and identify). If you need/want to put the digestion...

11. A Guide to Getting Started in Undergrad Research

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    Blog Post

    ...and responsibilities can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants...

12. Sequencing Options for CRISPR Genotyping

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    Blog Post

    ...mismatches and cleave them. The results are then visualized using gel electrophoresis. Surveyor™ and T7E1...

13. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ...available from commercial vendors. These libraries usually contain 4-12 gRNAs/gene, which results in libraries...

14. Typing CRISPR Systems

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    Blog Post

    ...effectors, like Cas9. The Cascade complex doesn’t usually carry out the function of the specific CRISPR type...

15. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post

    ...chromosomal integration modules, and many more of the usual suspects. The Multiplex Yeast Toolkit builds on ...

16. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    ...not be partisan: everyone (of every political persuasion) must be humble in the face of evidence. Stephanie...

17. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ...antigen interactions (Saraheimo et al., 2013), and visualizing structural changes in the ribosome during protein...

18. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post

    ...system, editing or labeling efficiency doesn’t usually change when you add multiple gRNAs on one plasmid...

19. CRISPR 101: Cytosine Transversion Editors

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    Blog Post

    ...this “non-target strand.” To avoid confusion, we usually use different terminology when discussing base ...

20. Promoters

    Type

    Guide

    ...functionally equivalent called the Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal... span a wide range of DNA sequences. It is not unusual to have several regulatory elements such as enhancers...