We narrowed to 421 results for: SUA
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TypeBlog Post... Genetic Engineering Era: Pre-CRISPR Having visualized and understood the DNA, the next milestone for...
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FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...to cell-type specific promoters; however, those usually drive weak expression of downstream genes. Strong... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post...differences. The original creators of this technique visualized the ligation product in a gel, but we can now... -
Quick Guide to All Things Lentivirus
TypeBlog Post... will first need to transfect producing cells, usually 293T cells, with your transfer plasmid and your... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post... digestion products (which could be easier to visualize and identify). If you need/want to put the digestion... -
Transferable Skills Guide: Teamwork
TypeBlog Post...experiments I’d been learning about for years were usually the products of teams: Watson, Crick, & Franklin... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...mismatches and cleave them. The results are then visualized using gel electrophoresis. Surveyor™ and T7E1... -
CRISPR Guide
TypeGuide...Overview of visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify Genomic...unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target... become a flexible tool to activate, repress, visualize, and isolate genes. Activation or Repression of...modification using CRISPR Browse Plasmids: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging...multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live cells. Labeling... painting, and RNA tracking in living cells. Visualizing a specific genomic locus requires recruitment...Chromosome-specific repetitive loci can be efficiently visualized in living cells using a single gRNA that has ... -
Typing CRISPR Systems
TypeBlog Post...effectors, like Cas9. The Cascade complex doesn’t usually carry out the function of the specific CRISPR type... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...chromosomal integration modules, and many more of the usual suspects. The Multiplex Yeast Toolkit builds on ... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...available from commercial vendors. These libraries usually contain 4-12 gRNAs/gene, which results in libraries... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...not be partisan: everyone (of every political persuasion) must be humble in the face of evidence. Stephanie... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...antigen interactions (Saraheimo et al., 2013), and visualizing structural changes in the ribosome during protein... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...this “non-target strand.” To avoid confusion, we usually use different terminology when discussing base ... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...system, editing or labeling efficiency doesn’t usually change when you add multiple gRNAs on one plasmid... -
Lentiviral Vector Guide
TypeGuide... Plasmid Interchangeable; usually encodes for VSV-G Interchangeable; usually encodes for VSV-G Safety ...Envelope plasmid — contains packaging gene env (usually VSV-G due to wide infectivity) VSV-G (vesicular... -
Promoters
TypeGuide...functionally equivalent called the Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal... span a wide range of DNA sequences. It is not unusual to have several regulatory elements such as enhancers... -
Gamma-Retroviral Vector Guide
TypeGuide...Envelope plasmid — contains packaging gene env ; usually VSV-G due to wide infectivity VSV-G (vesicular ...LTRs and replaced with a heterologous promoter (usually CMV or RSV). 5' LTR in cis Acts as an RNA pol II... -
Molecular Biology Reference
TypeGuide...type of horizontal gene transfer (conjugation), usually providing a benefit to the host, such as antibiotic... can be used to select or sort the cells by visualization or FACS). Primer Binding Site A short single-stranded... -
Plan Your Experiment
TypeGuide... restriction enzymes for further customization Usually paired with a sequencing method Can’t provide specific...