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We narrowed to 672 results for: abo.1

Showing: 401 - 420 of 672 results

  1. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...depths (1 mm), important for certain applications such as in vivo tissue imaging.   Figure 1: Ace-mScarlet...especially essential. If you want to learn more about how viruses can be used for chemigenetics, biosensors...

  2. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...principles of fluorescent dyes/ligands Figure 1: Example structures of fluorophores, fluorescent dyes... dopamine D2/D3 receptors. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-78827-9 Breen... Biophysica Acta - Biomembranes (Vol. 1838, Issue 1 PARTA, pp. 15–33). https://doi.org/10.1016/j.bbamem... A., Purkayastha, N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020...

  3. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...a ‘plasmid’. “p” is for plasmid. pXXXXX-XXXX Step 1: Backbone name Include the empty backbone name in ...Database. Step 2: Insert name  Include information about the insert in your plasmid name. This is often a... just by reading the name. For more information about plasmid features and tips you can use in the lab...

  4. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, but you’ve earned bragging ...need more convincing, I’ve got just the proof: Above our office team shows their love of plasmids! Each...

  5. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...alternative detection methods.      Figure 1: The four types of ELISA are direct, indirect, sandwich...conjugated antibody is not readily available. Table 1: Overview of the advantages and disadvantages of each...conjugate antibodies themselves, the process can be laborious and expensive. In some cases, conjugation methods...

  6. Hot Plasmids - November 2023

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    Blog Post
    ... depending on the specific edit.   Figure 1: Choosing the best PE6 variant for your application...modular envelope design. Nature Communications, 14(1), 5141. https://doi.org/10.1038/s41467-023-40788-8...section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts, including...

  7. Podcast: Interview with Addgene's Founders

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    Blog Post
    ...Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders? Chapter 2 - The process...repository’s future. If you’ve ever been curious about what it takes to start your own nonprofit, this ...Harvard University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge...

  8. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...light-sensitive LOV2 domain of Avena Sativa phototropin 1 (AsLOV2). In the dark, this peptide is caged by the...iLID nano and 800nM to 47µM for iLID micro; Figure 1). With this great affinity range, these LIDs have ...Figure 2). They are all available now at Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4):507-17....Guntas et al., Proc Natl Acad Sci USA 2015 Jan 6; 112(1):112-7. doi: 10.1073/pnas. CRISPR-Cas9 optogenetic... al., BMC Molecular Biology. 2013 August 20th; 14(1). TRICK: A method for visualizing the first round...for a variety of experimental purposes including (1) single or multiple, constitutive or inducible, gene...the years. The authors optimized these systems by: 1) decreasing the number of plasmids required for each...

  9. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ...by insertion and deletion frequency. Fig. 1: Overview of TIDE. The input is sequencing trace files...keep your product to knock-in size ratio below 10:1. Small knock-ins Is your knock-in edit small, with... your edit, it’s important to know a few things about your target cells and the desired edits. First, ...et al., NAR). It works just like TIDE (outlined above), except that it uses a 3rd sequencing trace file...great option, especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing...higher probability locations. If you’re worried about off-target effects, you can consider using a version...SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post here. Dealing with...

  10. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...great advice on all of these topics. Listen to Part 1 Here Listen to Part 2 here! Time : Topic 0:00 - 0:...How did you hear about post grad opportunities and when did you start thinking about them? 18:30 - 19:... top if you leverage your community, think hard about picking a good mentor, and begin considering career...19:54 : How to think about picking a post-doc project 19:55 - 22:40 : Improvements that can be made to ...

  11. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...activity to a transcriptional activation domain (Fig 1). Two ways to use CRISPRa for your research CRISPRa... Stucka R, Hauber J, Lange UC (2016) Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional... organisms (Chavez et al., 2016). To learn more about the different types of Cas9 activators, check out...covering VP64, Suntag, SAM, and VPR. Worry less about off targets Unlike Cas9 cutting activity, off target...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... Variant for Your Next Experiment Find out More about Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR...

  12. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

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    Blog Post
    ...school to a startup and back to academia. Chapter 1: How working at a startup prepared Tom for an academic...synthetic yeast chromosome. Listen to learn more about Dr. Ellis, synthetic biology, synthetic chromosomes...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder...BioBuilder Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with...

  13. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Figure 1). Clustered events are called a population.                             Figure 1: While...series of one- or two-marker plots, like in Figure 3 above, until the population of interest has been successfully...  Of course, there are many more things to learn about when diving into flow cytometry data. There’s different... ways to use FSC and SSC, as briefly alluded to above. There’s many variations in how one can gate, select...

  14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...single neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP) and...genOway press release for more information. References 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M. Sternson... on the Addgene Blog Learn More about FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs...expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and...

  15. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...folding, and deal with sub-optimal codon usage (Table 1) A few mutations are common to all or most expression...curated list of Bacterial Expression Systems Table 1: E. coli expression strains  Note: All strains are...How does inducible expression work? As mentioned above, many expression plasmids utilize inducible promoters...see protein overexpression? The strains described above should generate sufficient expression levels for...may help with solubility issues. We want to hear about your favorite protein expression tips and tricks...

  16. Addgene's Website Update

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    Blog Post
    ...straight to your materials of interest!   Figure 1: The new search bar.   Pro tip! You can use a manuscript...straight from the new search bar, but the menu just above it can help you quickly and easily navigate to educational...down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit, request...

  17. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout...cells are avaliable for distribution. To learn more about pooled library screening, read our recent blog post...the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common...

  18. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small self-structured...takes a lot of time and energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags...Pubmed PMID: 23435261. Resources at Addgene Read about other Protein Tags in our Plasmids 101 Series. Get...

  19. Antibody Guide

    Type
    Guide
    ...and placed in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made... together to form a “Y” shape, as shown in Figure 1. The two arms of the Y structure are responsible for...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...

  20. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...PGC-1 plasmids from Addgene. References: Skeletal muscle PGC-1α1 modulates kynurenine metabolism and...Institute on Aging in a commentary about the new work published in Cell Metabolism. But, they note, there is plenty...diseases.” I don’t know about you, but I think I could use a good run right about now. And, if you are looking...be important to identify the key proteins and metabolites that are dynamically secreted by muscle during...
Showing: 401 - 420 of 672 results