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  3. Colony Formation Titering Assay

    Type
    Protocol
    ...titer of your lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...All of the cells in the untransduced (negative) control well should be killed and no colonies should be...appropriate antibiotic. When the untransduced (negative control) cells have died and colonies are visible in the...

  4. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...insert. It is also important to set up negative controls in parallel. For instance, a ligation of the recipient...plasmid alone plate. The recipient plasmid alone control will tell you your “background” level or more specifically...see any colonies, you should conduct a positive control to ensure that your transformation worked. You ...depending on the number of background colonies on your control plate (the more background, the more colonies you...

  5. Rett Syndrome

    Type
    Collection
    ... in a new window) RSRT iPSC lines and isogenic controls , patient-specific Infinity BiologiX - RSRT cell...

  7. The Challenges of Cell Culture

    Type
    Blog Post
    ... laboratories to carry our independent quality control for a range of testing, from animal doping to forensics...

  10. MXS Chaining

    Type
    Blog Post
    ...meaning that (in the previous example) one can control whether the third module is added to the 5’ terminus...

  19. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins...
Showing: 401 - 420 of 484 results