We narrowed to 494 results for: control
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TypeBlog Post...of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ...
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Colony Formation Titering Assay
TypeProtocol...titer of your lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...All of the cells in the untransduced (negative) control well should be killed and no colonies should be...appropriate antibiotic. When the untransduced (negative control) cells have died and colonies are visible in the... -
Plasmid Cloning by Restriction Enzyme Digest (with Protocols)
TypeProtocol...insert. It is also important to set up negative controls in parallel. For instance, a ligation of the recipient...plasmid alone plate. The recipient plasmid alone control will tell you your “background” level or more specifically...see any colonies, you should conduct a positive control to ensure that your transformation worked. You ...depending on the number of background colonies on your control plate (the more background, the more colonies you... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...not that I would know about this… A no template control (NTC) should always be included. While the physical... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic... -
Plasmids 101: Codon usage bias
TypeBlog Post...expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...little enrichment of DNA targeted by a negative control gRNA or predicted gRNA off-target sites. Long-range... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...ladders from Penn State University Use Nanobodies to Control Protein Activity Learn more about protein tags ... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...construct is Cre-dependent, enabling spatiotemporal control over its expression. Constitutive or inducible ... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...as an RNA-guided platform for sequence-specific control of gene expression." Cell 152.5 (2013): 1173-1183... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...modifications in the same strain. MazF expression is under control of the AOX1 promoter whose expression is induced... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...FRET in Your Experiments Learn more about GFP Controlling Protein Activity with GFP Resources at Addgene.org... -
MXS Chaining
TypeBlog Post...meaning that (in the previous example) one can control whether the third module is added to the 5’ terminus... -
Hot Plasmids: Summer 2025
TypeBlog Post...non-selective anterograde transport to a lack of genetic control. ATLAS (anterograde transsynaptic label based on... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post... messy, and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the... -
The Challenges of Cell Culture
TypeBlog Post... laboratories to carry our independent quality control for a range of testing, from animal doping to forensics... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...use of viruses allows much better spatiotemporal control over labeling methods as compared to transgenic...