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We narrowed to 508 results for: dna plasmids

Showing: 401 - 420 of 508 results

  1. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...Novel techniques for the prevention of mitochondrial DNA disorders.” This document is about the popular technique...Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Check out our genome engineering guide...scientific possibility, safety, and efficacy of the mtDNA transfer options being investigated; A UK regulatory...

  2. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transducing primary cells. What if, instead of supplying a DNA construct, they could simply induce cells to take...10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters...

  3. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR... by a particular gRNA. In the enChIP (engineered DNA-binding molecule-mediated ChIP) system, catalytically...catalytically inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag or tags can be fused...Browse, sort, or search the tables below for CRISPR plasmids designed for the purification of genomic loci....

  4. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...in the DNA, possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...transcription none, need Cas9 plasmid Chen Do you have suggestions for other plasmids that should be added to...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in Xenopus. Cut Fully...

  5. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance... Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...CRISPR plasmids that have been designed for use in zebrafish. CRISPR... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional ...

  6. CRISPR Plasmids - Parasites

    Type
    Collection
    ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...expression plasmids that have been designed for use in parasites. CRISPR... CRISPR Parasites CRISPR Plasmids: Parasites...Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional ...development. This collection brings together some of the plasmids that have been used in these efforts. The content...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss ...

  7. Rett Syndrome

    Type
    Collection
    ...CRISPR-based DNA methylation editing system - Shawn Liu and Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...Rett Syndrome Rett Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR... CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) ...individual laboratories to assemble a Rett Syndrome plasmid resource for the scientific research community....

  8. Deep Dive: qPCR

    Type
    Blog Post
    ...single-stranded) DNA. As the temperature drops, single intensity should smoothly increase as the DNA amplicon ... are produced by the presence of double-stranded DNA, when temperatures are high, signal intensity should...dyes that are not dependent on amplification of the DNA; they are, however, affected by bubbles in wells,...LightCycler qPCR optimisation for low copy number target DNA. Journal of Immunological Methods 270 (2002) 119 ...is a smooth, single-step transition between ssDNA and dsDNA. This is not true for all amplicons; if your... probes (which you probably know as TaqMan) and dsDNA dyes (commercial name: Sybr). However, there are... Yes Yes Agarose gel or uMelt analysis dsDNA dye (Sybr)  High No No No Melt curve ...

  9. AAV Packaged on Request

    Type
    Collection
    ...transfer plasmids in our repository are eligible for this service. Select any eligible plasmid in the Addgene... for the transfer plasmid, including legal approval and an implementation letter DNA amplification Viral...applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the providing...real-world troubleshooting. Eligible Plasmids The following plasmids are eligible for this service. Submit...paper of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter ...viral vector preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures...Overview Details Ordering Share Your Results Eligible Plasmids Additional Resources Adeno-associated virus (AAV...

  10. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...... Restriction Digest of Plasmid DNA Purifying DNA from an Agarose Gel DNA Ligation Introduction Gel ...length of a DNA fragment by running it on an agarose gel alongside a DNA ladder (a collection of DNA fragments...negatively charged DNA through an agarose gel matrix toward a positive electrode. Shorter DNA fragments migrate...100 mL gel). EtBr binds to the DNA and allows you to visualize the DNA under ultraviolet (UV) light. Caution...from the DNA. So if you run the gel without EtBr in the buffer you will reach a point where the DNA will ...that has UV light, visualize your DNA fragments. The fragments of DNA are usually referred to as ‘bands...or c) loading less DNA into the well. Another method for visualizing very short DNA fragments is polyacrylamide...

  11. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols...of two targets independently. The RNA-programmed DNA-binding protein dCas9 is expressed along a CRISPR...expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The first one...18, or 20 matching nucleotides. Together, these plasmids allow to explore the whole range of expression...amount and stoichiometry of enzymes. The pLC97 plasmid allows for quick integration of the aTc-inducible... One-Step cloning and chromosomal integration of DNA. ACS Synthetic Biology, 2(9), 537–541. DOI: 10.1021...

  13. Common Injection Routes in Mice

    Type
    Blog Post
    ...Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo...substances can be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers must...

  14. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid Calarco... need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem 47912...elegans none, need Cas9 plasmid Ristow Do you have suggestions for other plasmids that should be added to...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...CRISPR plasmids that have been designed for use in C. elegans. CRISPR...

  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...creates the DNA double-strand break (DSB) at the target site, and a donor oligonucleotide or plasmid as the... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting..., the gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected...provided (in the form of an oligonucleotide or plasmid), precise changes can be made in the genome via...mice carrying the intended mutation. With a donor plasmid, the outcome is less predictable. In our best case...

  16. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... be used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies... target protein. However, small mutations in the DNA of the hybridoma can occur, known as genetic drift...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...protein (Wang et al., 2013). These proteins are also plasmid-derived. However, a disadvantage to ScFv is their...nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common internal control ... as well as tools for developing and producing plasmid-based antibodies - and we’re always growing our...

  17. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...meaning “to fit”. SELEX could be used to classify DNA aptamers as well, and over the course of the next...

  18. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... FRET-based biosensors, and interactions between DNA and proteins. FRET, in theory, is an easy technique...used pairs to measure FRET. The table below lists plasmids that can be used to create your choice of fluorescent... fusion protein with your gene of interest: Plasmid Color Expression Description pPROEX Aqua Cyan...

  19. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...cite the original paper but link to plasmids from the Promega Plasmid Collection that express similar constructs...Addgene Luciferase 101 Luciferase Plasmid Collection Promega Plasmid Collection ...difficult-to-study genes. Its main function is to regulate DNA repair, senescence, and apoptosis. Work by Kamio ...cell and protein analysis products. You can find plasmids deposited by Promega scientists here.  References...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target cells' genomes... ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out Our Cancer Resources Page...
Showing: 401 - 420 of 508 results