We narrowed to 883 results for: prot

...readouts for levels of different chemicals, hormones, proteins, etc., that you are interested in. The most obvious...includes tension monitors associated with specific proteins of interest such as actinin. When tension is high....2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service: Biosensor AAV Viral...Epigenetics with a FRET Based Biosensor Fluorescent Proteins 101: Fluorescent Biosensors ...

...helping us establish consistent, high-quality protocols for lentiviral production. Bryan Roth’s lab from...technical service (details about virus production protocols and quality control can be found on Addgene’s ...and Office Teams develop shipping systems and protocols for viral vectors to reach scientists around the...Beginners Resources at Addgene.org Find Virus Protocols Visit Our Viral Vector Pages Find Virus for Your...

...that occur during DNA synthesis, and protomoting or repressing protein expression. Methylases involved with...restriction modification systems that prokaryotes use to protect themselves from foreign DNA. The other component...Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...

...indel generation for two PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing...3 and 31 times higher, respectively. In rice protoplasts, Xie et. al observed slightly higher mutation...mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How many loci can...gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs targeted genomic sites separated...

...Activation Mediator or SAM. SAM is an engineered protein complex for the transcriptional activation of endogenous... plasmid which expresses the activation helper protein. SAM can be combined with a human genome-wide library...spot to fuse the activators instead of at the protein’s C terminus. With that new design in place, Konermann... one time. Weissman’s group has made standard protocols available and they are working to further optimize...

...mammalian cells, especially when fused to effector proteins for gene activation or gene editing. The more ...various delivery methods, including plasmid, ribonucleoprotein, and AAV.  Read the paper in Nature Chemical...delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own mRNA to facilitate...RNA motif. These engineered pegRNAs (epegRNAs) protect the RNA from degradation by cellular exonucleases...

...possibly inducing the secretion of growth promoting proteins, such as human growth factor, vascular endothelial...strong in this one. After all, the system built to protect us now seems to attack us by releasing mitogenic...microenvironment itself. They deliver compounds like proteins and nucleic acids from cancer cells to the microenvironment...exosomes expressed specific integrins (cell-adhesion proteins) targeting different organs. This means that exosomes...

...create an almost limitless number of DNA binding proteins with Cas9 guided by different promoters. The challenge... genetic circuits is getting enough regulatory proteins that don’t interfere with each other. CRISPR is...much easier it is to work with than regulatory proteins. There are still things we haven’t solved that...Nothing too much more sophisticated. There are also proteins, like antibodies. But we are really just using...

...composed of a tightly-bound invariant group of proteins, whereas the more loosely bound co-coregulators...explain how more than 300 different coactivator proteins individually contribute to cell regulation in ...coherent manner. Because coregulators exist as multiprotein complexes, a member of a single coactivator .... Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa cells was the...

...potential to bind many different sites on the target protein they tend to have a strong signal and to work in...mouse derived primary antibody to stain a target protein in mouse tissues, causes a high degree of background...sample. There are species-on-species staining protocols that use antibody fragments to block the endogenous...Biological systems tend to be complex with a variety of proteins interacting. Experiments tend to be equally complex...

...are a number of drawbacks to this approach: TET proteins are not demethylases; they are dioxygenases that...rare DNA bases that are recognized by distinct proteins and result in unclear transcriptional consequences..., Charlton et al. 2020). Most importantly, TET proteins affect transcription independent of their enzymatic...may seem counterintuitive: how can a DNA-binding protein with no enzymatic activity cause demethylation?...

...once. Fibronectin is another membrane-interacting protein that can be used to enhance transduction efficiency...Alternatively, if the virus encodes a fluorescent protein, the packaging cells could be examined with a microscope... While there may be some low-level fluorescent protein or resistance marker expression following transfection...Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ...

...drawbacks of different methods (for comprehensive protocols for all of the methods discussed here refer to...and are usually based on the presence of a viral protein, such as p24, or viral nucleic acid. Functional...staining. Check Out Our In-Depth Fluoresence Titering Protocol Another popular approach utilizes the antibiotic...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2....

...remodeling. Chromatin is a complex of DNA and histone proteins that compacts cellular DNA into chromosomes and...localise the construct specifically to the histone protein of interest (in the figure above, H3 at K9 or K27...Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors Read Our Tips for Using FRET in Your...Experiments Post Read Other Blog Posts about Fluorescent Proteins  ...

...boundaries defined by anti-CRISPR proteins or running into other proteins naturally bound to the DNA. Cas3...Type I-E Cascades contains all five of these Cas proteins, while type I-C Cascades do not contain Cas6. ...Cas9 vs. The Other Cas(s) CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems INTEGRATE: Bacterial...

...details on this procedure check out our PCR protocol page, protocol video and reference on how to design PCR...Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer  ...

...to tag all 22,000 protein coding genes in the human genome with fluorescent proteins. For each gene, we...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...outwards so that the DNA sequence is altered but the protein sequence they encode is not. After each mutation...

...thinking of an amazing project. You’ve written your protocols, designed your experiments, and prepared your ...purifications, or ligations. The general Gateway cloning protocol is fairly flexible and adaptable to cloning different... they will come with comprehensive and tested protocols, as well as premixed and ready-to-use reagents...101 eBook Resources on Addgene.org0 Check out protocols for plasmid cloning Find vector backbones Read...

...previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in of a GFP-Puro cassette...much shorter sequence homologies. Abbreviated protocol for PITCh Step 1: Generate microhomologies in ...TALENs and CRISPR-Cas9 with the PITCh systems.” Nat Protoc. 11(1): 118-33. PMID: 26678082. Find the plasmids...Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621...

...construct on the same piece of DNA. We do a lot of protein-protein interaction work and we’ve gotten some tools...contains sequences for several different fluorescent proteins. Visualizing fluorescence can be challenging in...chlorophyll, so we looked at newer fluorescent proteins that get around that problem. MoClo is really ...