We narrowed to 405 results for: pho;
-
TypeBlog Post...Screening Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 ...
-
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate... Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed cell... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post... This process can be avoided direct antibody fluorophore conjugates are used, but these are usually more...secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization is... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...basic science research (neuronal tracing and morphologic studies), as well as translational applications...capsids are useful tools for neuronal tracing and morphology studies when used as part of a two part fluorescent... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...your samples and maintaining cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker...reagents - cross-linkers better preserve sample morphology, while organic solvents can reveal otherwise ... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...The kan resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...Using a spectrophotometer is among the faster methods of quantification. Many spectrophotometers have an... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...recently purified MDH to study the impact of phosphorylation on the enzyme’s structure, function, and interaction... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...including a lot of future Addgenies as shown in the photo below. Kids and adults alike enjoyed some friendly...of your plans by email, gong, evite, posters, megaphone, or your company’s intranet. Don’t forget about... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...this unique sequence of DNA and both identified lymphocyte antigen 6 complex (LY6A) as a receptor for PHP.B...discovered as an antigen upregulated on activated lymphocytes and is commonly used to enrich adult mouse hematopoietic...