We narrowed to 545 results for: SEC

1. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   ...divide the viral elements among multiple plasmids. Second generation packaging systems use 3 plasmids; an...

2. Adenoviral Vector Production and Troubleshooting

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   Blog Post

   ... accommodate up to 8.2 kb of transgene DNA. The second generation vectors are deficient in E1, E2, E3,...

3. Rabies and Neuronal Tracing

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   Blog Post

   ...these high levels of viral replication are toxic. Second, infecting subsequently higher and higher order...

4. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   Blog Post

   ...embryology techniques such as basic observation and dissections. Students don’t usually perform more modern techniques...

5. A Look at Addgene's QC Process

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   Blog Post

   ... our collection. This standardization provides security for requestors, ensuring that the plasmid they...

6. Design Tips for Prime Editing

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   Blog Post

   ...different lengths and sequences, as unintended secondary structures in your pegRNA could inhibit editing...

7. Viral Vectors 101: Calcium Sensors

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   Blog Post

   ...fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural...

8. Plasmids 101: The Wide World of Natural Plasmids

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   Blog Post

   ...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...

9. Plasmids 101: Modular Cloning

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   Blog Post

   ...behaviors are available from Addgene and other sources; second, characterizing a part or optimizing a TU is much...

10. Progress Towards a PAM-Free CRISPR

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    Blog Post

    ...sequence, including propensity for off-targets, secondary structures, etc., as well as the presence of a...

11. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post

    ...luciferase, cre/lox, yeast expression, worm expression, insect expression, plant expression, mouse targeting, ...

12. Tips for Technical Support Calls

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    Blog Post

    ... At least at Addgene, the questions in our FAQ section are really, truly frequently asked by customers...

13. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post

    ...virtually any RNA sequence (Green et al, 2014). The second was an in vitro paper-based platform that allowed...

14. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post

    ... to cleave again and possibly mediate HDR in a “second chance” mechanism. To prevent new editing post-...

15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post

    ...Laureates and the ever expanding importance of RNA The second keynote lecture was presented by Nobel Laureate...

16. The Materials Science of Optogenetics Experiments

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    Blog Post

    ...preparing the implant itself, which brings me to the second key point; the fiber optics need to be polished...

17. Gaining Leadership Skills Volunteering at a Professional Organization

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    Blog Post

    ...as things came up. For example, I updated some sections of the NPA’s website. By volunteering, you can...

18. How-to: 5 Steps to a Great Panel Discussion

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    Blog Post

    ...things rolling. It can make or break the discussion section. And what about those audience members that want...

19. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    Blog Post

    ...using biotechnology) were thrown on the table and dissected with equal enthusiasm. It may have started as ...

20. Of Myc and Men

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    Blog Post

    ...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...