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We narrowed to 922 results for: POR

Showing: 631 - 660 of 922 results

  1. Hot Plasmids - February 2022

    Type
    Blog Post
    ... provides a useful yet low-pressure teaching opportunity for lab members or students new to protein expression...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

  2. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ...

  3. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...NPR, articles in magazines like Fast Company, opportunities to speak at conferences, and offers for postdoc...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

  4. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...the target cell or transiently expressed. It’s important to note that for all the applications we discuss... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the...

  5. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using... substrate is required for imaging, and it is important to verify that this substrate does not alter cellular...

  7. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...detection limits of about 5% mutant targets have been reported for mismatch nuclease assays (2, 6, 7). Ability...nuclease assays are completely “blind” to this very important difference (Figure 3). If you are interested...

  8. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...from the mol bio side you’re sure to have many opportunities to learn about and develop technologies that...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...

  9. Clear and Accessible Writing

    Type
    Blog Post
    ...reader would glean from the image itself.  As we incorporated alt text into our work, we began to realize ..., and we’d love to hear yours as well.  More importantly, you can use these four steps for any type of...

  10. How Addgene Creates Content

    Type
    Blog Post
    ...conversations, conferences, and questions our scientific support staff receives – in fact, the blog originally started... post (email [email protected].) We are here to support and advance the scientific community, and we can...

  11. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...aging. For both a screen and a selection, it is important to clearly define what type of factor you are ...whole genome), they knock down gene expression temporarily. RNAi libraries are especially useful if you ...

  12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...sequence flexibility indicates that gRNAs could be incorporated into these constructs without compromising cleavage...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

  13. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...process involve repairing chromosomal DSBs. It is important to know that cells possess two major repair pathways...chance of causing a frameshift mutation. Of some importance, the deletion can be less heterogeneous when ...

  14. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...): These are considered low copy vectors and incorporate part of an ARS along with part of a centromere...nutrient, the host cells will die unless they have incorporated the plasmid carrying the required gene.  The...

  15. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...plasmids have been introduced by transfection or electroporation. All you do to create a protein that binds ...where particular variants or mutations have been reported in people, we can use this kit to knock in those...

  16. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...ain't broke, don't fix it!  That being said, it's important to know all your options when planning and running...

  17. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...for IgG subtypes from different animals so it’s important to choose the reagent that works for your species...sure the staining you are seeing is real. It’s important to include a negative control using serum from...

  20. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interacting proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster...identify highly biotinylated background proteins reported by other research groups that performed BirA*-...

  21. To Each HIS Own

    Type
    Blog Post
    ...tended to be very large and often required the incorporation of a cleavage site and a post-purification cleavage...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...

  24. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...be helpful having a coach that can offer extra support for issues of diversity in science.  A great person...

  25. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...500ML Benzamide, Millipore Sigma 12072 Antipain, Millipore Sigma 10791 Leupeptin, Millipore Sigma L2884 Aprotinin...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. General Considerations... Aprotinin saline solution, Millipore Sigma A6279 Before Starting Warm the DNA and working stock of PEI-MAX...

  26. Hot Plasmids - October 2020

    Type
    Blog Post
    ...grown in high concentrations of IL-3 show a high proportion of blue fluorescence, indicating cell proliferation...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...

  29. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...
Showing: 631 - 660 of 922 results