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We narrowed to 1,076 results for: TOR;

Showing: 631 - 660 of 1076 results

  1. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...biodistribution therefore introduce additional confounding factors when attempting to interpret signals from these...collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...years to clinically treat enteric infection and inflammatory bowel conditions. In these experiments, arg1...

  2. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...match sequence information contributed by the depositor, published backbone sequences, and any known sequences...analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps...experiments like restriction digests. We’ll have a tutorial showing all of the features available on our Snapgene...

  3. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...optogenetics blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org...

  5. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...individuals through stock or investor pay outs. Addgene has no investors or stock. All of our proceeds...proceeds go into running the repository and serving scientists better. That includes keeping fees as low as ...

  6. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...biological sensors’) are biological tools that monitor a process or detect a given molecule. The sensor...in. Depending on the process you would like to monitor, there may be multiple steps you could examine,...biosensors. These constructs are often used to monitor enzymatic activity or phosphorylation over time...

  8. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...original policy was straightforward, but cumbersome: depositors had to email us with permission to release their... are left in the hold status and reach out to depositors about them, but we all know how easy it is to...your work. Plus, it’s costly for us to process and store materials that aren’t distributed or for our teams...

  9. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...affiliated TniQ protein recruits transposition cofactors. The transposon proteins integrate a payload sequence... Figure 1: Mechanism of CAST systems. A Cas effector (Cascade in Type I systems; Cas12k in Type V-K...Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase. Science...

  10. An Addgene Summer Internship

    Type
    Blog Post
    ... gene in a pLenti vector backbone. During those five weeks, I worked with my mentors, Katherine, Amrita...exchanged contact with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’...

  11. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...array: The section of the bacterial chromosome that stores a genetic record of previously encountered foreign...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

  12. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...department.” Caitlin does this by acting as a facilitator between her team, the sales department, and operations...business.” Caitlin’s advice for budding science communicators: “My recommendation would be to take any opportunity...education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden Get tips...

  13. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...For this series, I’ll interview three science communicators who work in the biotech, education, or nonprofit...job. “I am really more of a multimedia science communicator than a science writer these days. I do some ... to talk to other scientists when I am in new territory. Perhaps there are examples of science writers...

  14. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...patient partners so that they can help tell their stories. I was paired with a six-year-old boy named AJ ...caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration...where my wife Lianna has made her career as Senior Director of Biology, is helping us fight against FOP in...

  15. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...you questions. Remember that people like a good story and science involves characters – in this case you...or other places online, and consider how those stories are being told. Twitter can be a good way to follow...sharing – both your plasmids and your research stories. Consider practicing communicating your research...

  17. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...meaningful day job only came into focus when an English tutor noted my eclectic mix of science and arts papers...venturing forth from tertiary study, the myriad trajectories ahead can be condensed into two discernible ...truly benefit others. Herein, a good science communicator—with an understanding of how to appeal to a ...

  19. Plasmid Cloning by PCR

    Type
    Blog Post
    ...will describe how to copy a cDNA from one vector into a new vector that is better suited for analyzing the...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

  20. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    .... I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge and knew...outside of academia, a job as a ‘Cancer Marketing Coordinator’ came up at Abcam. Unlike other job descriptions...knew there would be a way around this. First off I tore up my “Scientific CV” and wrote a more business ...

  21. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...who may not be experts, can you give me a brief history of optogenetics? How long has this idea been around...optogenetic tools for neuroscience back in 2005, both activators as well as silencers of neural activity. Since...brain is very complex and we've hosted hundreds of visitors in our lab to help them understand their specific...

  22. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...great deal more information that one might like to store about FPs than is currently represented at fpvis...a centralized, community-editable resource for storage and dissemination of information about FPs. FPbase.org...elaborate spectrum analysis tools, and a better way to store and represent within-study photobleaching comparisons...

  23. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...classification system, including its history, biology, and usages in the lab. History In 1884, while studying infected...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

  24. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination in cultured...control the CRISPR system activity.  Using an AAV vector may help with size limitations. In addition, non-viral...

  25. Hot Plasmids - January 2023

    Type
    Blog Post
    ... plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think we missed...expression of the tumor-seeking CAR (chimeric antigen receptor), whereas a ubiquitous promoter drives expression...can be made for expressing your favorite T-cell receptor, or perhaps any other tool that can benefit from...

  26. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...interactions and growth within the biofilms will then be monitored using fluorescence microscopy and FACS. In an ...to create gene deletions of important unknown regulatory regions in Pseudomonas and Klebsiella spp. The...projects to reach audiences beyond our plasmid depositors and requesters. Check out some of our recent ...

  27. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 ...which causes the breaks) and host cell DNA repair factors (which repair those breaks) makes Cas9 extremely... optional donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating...

  28. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University...Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org...

  29. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...Excellent scientists learn from advisers and other mentors by collaborating as they craft sentences for abstracts...in all STEM fields become excellent science communicators. To empower graduate students to become ambassadors...careers in science communication The strength of story telling Science communication: 9 strategies to get...

  30. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...one to study RNAs expressed from their native regulatory elements. Controls for RaPID experiments RaPID...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...RNA-binding protein that’s highly expressed in neural progenitor cells. Knock-down of QKI decreased Zika viral...
Showing: 631 - 660 of 1076 results