Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,029 results for: ELL

Showing: 841 - 880 of 1029 results

  1. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... a result, the materials in our catalog are very well-characterized. During this time, we’ve also created...improved menus we launched earlier in the year, as well as our redesigned homepage and our new search tool...suggestions provide shortcuts to specific catalog items as well as related items (for example, a kit that contains...

  2. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... really starting from the beginning. Luckily, a fellow graduate student and a data analyst in our lab ...dig in to the computation, but they will serve you well as you perform analysis after analysis after analysis...However, these notes take up a lot of space and don’t tell you why you did something or what the result was...

  4. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this...tried-and-true protein expression control tools as well as explore some new innovations. Whether you want...tag prior to introduction of the construct into cells. However, if you would like tunable control of an...

  5. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...produce results, not scientists”  These comments are well correlated with published data. For example, in ...following panel members: Jonathan Dinman, Chair of the Cell Biology and Molecular Genetics Department at University...24733905. Pubmed Central PMCID: PMC4000813. 2. McDowell, Gary S., et al. "Shaping the Future of Research...

  6. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...enters in the middle of the action. If the paper is well-written, the abstract should give you a sense of... a few different ways to automate the process as well. Sign up for eTOC (electronic table of contents)...searching and sorting your own digital library as well as some integration with internet-wide literature...

  7. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ... PAM constraints just won’t cut it anymore. Say hello to PAM-free and PAM-flexible nucleases! In this ... for off-targets, secondary structures, etc., as well as the presence of a Protospacer Adjacent Motif ...Precise and efficient scarless genome editing in stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI...

  8. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...generated beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work...the most common poster size, but this can vary as well.  Sections of your poster In almost all scientific...there are some particular styles recognized to work well. It is important that your readers can navigate ...

  9. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...advice is always "don't do it unless you can do it well" – even the smallest program. I don't mean have ...have all been there. A good panel discussion is a well choreographed performance with room for fortuitous..., but the organizers and moderator should have a well-planned approach to making the event interesting...

  10. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...visible whether expressed in E. coli, in mammalian cells, or in purified form, and didn’t need any added ...like quantum chemistry and protein biophysics, as well as inventors and biomedical engineers, join efforts...References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants, B. W., & Robinson...

  11. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...held your favorite protein in the palm of our hand? Well, actually – have you ever held a model of your favorite... the real thing. Nevertheless, the models are compelling, and I encourage you to hold one. Why do we ...protein might represent one aspect of the protein very well, it will almost certainly fail to convey other aspects...

  12. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...It is a well known fact if a multicopy variant of a gene exists, there must be assays that researchers...the genome that are difficult to accurately read (Bell, 2018.) Even though it does not require a standard...Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll SA. Analyzing Copy Number...

  13. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ... by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and Harvard Medical School...with appropriate flanking homology into a germline cell can create an RNA-guided gene drive. In principle... experiments2,3. While that didn't work quite as well as anticipated4, greater awareness will certainly...

  14. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...new pipette tip for each step and mix the samples well by vortexing or inversion. Created with BioRender.com...values and the predicate values and measures how well the data points fit the trendline. R2 ranges from...change pipette tips regularly, and mix samples very well.  Use duplicates or triplicates to increase the ...

  15. Tips for arabidopsis transformation

    Type
    Blog Post
    ...guidelines here. A detailed transformation protocol, as well as protocols for growing Arabidopsis are also available...incubated at stationary phase for too long, the cells in your culture will no longer be viable. The time...graduate student at Stanford University. She studies cell fate maintenance in the stomatal lineage and has...

  16. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... . Steps of an IP: 1) Protein extracts from cells or tissues contain a complex mix of proteins seen...common immobilization methods utilizes bacterial cell wall proteins, Protein A and Protein G, which bind...does not. Common negative controls include knockout cell lines or tissues that do not express the protein...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...others may feel they can share opposing opinions as well. I am sure we have all experienced a meeting where...stop you from speaking up.  If your ideas are not well received, understand that people can be resistant... as many solutions as you can in that timeframe. Tell yourself they don’t have to be perfect or even good...

  18. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...attend. Societies, such as the American Society for Cell Biology (ASCB) or the American Chemical Society ...looking for work in today’s job market should have a well-groomed LinkedIn page. Here you can search for jobs...someone professionally that you do not know very well; always ask for information and establish a connection...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ... the 926 video abstracts identified. The journal Cell had the largest average view count per video abstract... on sharing your research through video. Liz Haswell and Eric Hamilton, biology professors at the University.../wesharescience.com/pin/7472 Article: http://www.cell.com/current-biology/abstract/S0960-9822(16)30873...

  20. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...But ask any filmmaker or videographer and they’ll tell you, a lot of the fun is building within the constraints...Some came in even lower.  Learn: While this works well for social media videos, we need as much detail ...stopping recording).  Test: It was unbelievable how well this worked! Interviewees found it easy to drop ...

  21. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...established theory. Realizing that textbooks don’t tell us everything there is to know is a strange and ...be the first person to make a discovery. I can’t tell anyone else what the “correct” motivation to do ...recommend talking to other undergraduate researchers, as well as graduate students (your teaching assistants),...

  22. Addgene Launches Partnership with IPI

    Type
    Blog Post
    ...to provide researchers with a collection of IPI’s well-validated and ready-to-use recombinant antibodies...of Addgene’s recombinant antibody collection, as well as access to IPI educational resources developed...

  23. CRISPR Guide Update

    Type
    Blog Post
    ...developed and widely adopted in the past few years, as well as refreshing the existing content. Access the CRISPR...more flexible requirements and enhanced editing, as well as alternatives to Cas9. All of this is accompanied...

  26. Deck the Lab 2022!

    Type
    Blog Post
    ...Sleigh bells, please! It's time to announce the winners of the 2022 #DeckTheLab contest!  and it's.....y34Tv8VCLT— Borris Aldonza (보리스) 🇰🇷🇵🇭 (@PrecursorCell) December 12, 2022          View this post...

  27. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...History Rap Battle A great example of science story telling following the unfortunate story of Rosalind Franklin...the African American woman whose cervical cancer cells became a mainstain of biological study without her...

  29. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...co-founder of Addgene. Melina received her Ph.D. in Cell Biology from Harvard University, where she studied...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

  31. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...publication, you'll find that information in the report as well.    Figure 3: An antibody data report, showing ...report, you’ll find a results section, which will tell you if the antibody worked under the conditions ...

  33. Addgene's Website Update

    Type
    Blog Post
    ...happens if you do scroll down? You’ll find Blugene, as well as information about Addgene, our mission, links...moment to let us know what you think, through the yellow popup at the bottom of the page. Happy surfing!...

  34. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ...include popular envelope and packaging plasmids as well as transfer plasmids. Download the orange lentivirus...instead, don’t fret; we have backgrounds for you as well! If you use FRET, you might be interested in our...

  36. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...multiple plasmids in one cell, it might be that you can't stably maintain them in the cell. This blog post features...

  38. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...written content about timely scientific topics, as well as archived seminar presentations. One of SITN'... In return, the public receives trustworthy, intelligible scientific resources. SITN hopes to make DayCon...

  39. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...
Showing: 841 - 880 of 1029 results