We narrowed to 877 results for: HA

...something called “the photon budget”: each sample has a limited number of fluorophores and each fluorophore...

...expression is sufficient. Lentiviral vector technology has come a long way since the 1990s, both in terms of...

...caveat that phages are very diverse, and each phage has a narrow bacterial host range. To further potential...

...measles, mumps, and rabies. Research on Ebola virus has been limited, in part, due to the necessity for working...

...enabled numerous cell biology assays. However, there has been a pervasive view that most FPs in a cell will...

...transfection reagents or the transfection process itself has any cytotoxic effects on the target cells. Another...

...sometimes put work ahead of life Sometimes, work has to come first. That means you and/or your spouse ...

...reports which POI band is stronger. The right gel has exactly the same POI bands as the left gel, but because...

...Looking forward Over the past twenty years, Addgene has made huge strides in increasing the accessibility...

...Addgene have worked hard to ensure that the service has grown steadily, helped by a partnership with the ...

...re-cutting the target sequence once the desired edit has been introduced. We found that blocking mutations...

...Bassik lab created a human knockout library that has 10 gRNAs per gene and targets all ~20,500 protein-coding...

...Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches to record keeping. ...

...problem or a need for something, usually someone else has realized this too. Begin with informal conversations...

...inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs and availability...

...effects, you can consider using a version of Cas9 that has been specifically engineered for reduced off target...

... It makes a great point when I then ask “and who has deposited to the repository?” When most or all of...

...Academies define a postdoc as “An individual who has received a doctoral degree (or equivalent) and is...

...means they don’t have the money. If the department has a strict budget, or a lack of resources, then ask...

... BL21 (DE3) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression than pLysS; expression...

...available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change (from...

...selecting the primary antibody host species, as it has to be different from the other antibodies in your...

...deprotonated state (Tsien, 1998). The deprotonated state has an absorbance maximum around 488 nm and it emits ...

...organization, because confirming that every person has read and understood every communication that gets...

...editors (ABE) What if you have another gene Y, which has a defective allele Ym and you need to convert an ...

...where the vector that you want to use is very large, has a high GC content, contains a lot of repeats--any...

...OpenWetWare's E.coli genotype resource. Additionally, NEB has a great list of genetic markers for your reference...

.... Precise, real-time pharmacology of this target has been hampered by transient interactions with co-receptors...

...ever thanks to the virtual world that the pandemic has foisted upon us. Action Items:  A simple place to...

...any immunogenicity of the AAV vector Once delivery has been optimized, it’s important to ascertain how long...

...methods. Each type of antibody and production strategy has advantages and disadvantages compared to each other...

...few commonly used fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use...

... These tags most often consist of an enzyme that has been mutated to block its catalytic activity and ...

...high contrast ratio usually indicates that a PA-FP has a low level of spontaneous fluorescence in the absence...

...multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use mammalian...

...your gel or blot, Table 1 (at the end of the post) has a number of helpful suggestions.  Gels Gel issues...

...collection . Protocols The UC Davis/NIH NeuroMab Facility has several protocols available that may be helpful for...