We narrowed to 1,006 results for: RING

1. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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   Blog Post

   ...software for your needs 5 great apps for lab life  Sharing your lab protocols using apps to save you time ...

2. Antibodies 101: Fc Effector Functions

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   ...bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent effector function known...

3. CRISPR 101: Drug discovery and CRISPR

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   ...Resources CRISPR History and Development for Genome Engineering    ...

4. Fluorescent Proteins 101: Fluorescent Biosensors

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   ...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...

5. SunTag and Fluorescent Imaging

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   ... Mary Gearing...

6. FastCloning: A Newer, Simpler Cloning Technique

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   ...plasmid to confirm no mutations were introduced during PCR or any subsequent steps. Using high fidelity...

7. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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   ... can’t be reused without attribution, despite filtering for images labeled for reuse with modification...

8. Understanding GPCR Signaling Using TRUPATH

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   .... The Roth lab used structure-guided protein engineering and experimental refinement to determine the ...

9. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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   ...supplemental files to see the actual data. Now you’re wondering if your favorite gene is included in other CRISPR...

10. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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    ...first collapse arg2 and then arg1 vesicles. By comparing ultrasound images pre-collapse and post-collapse...

11. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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    ... cry genes from the soil bacterium Bacillus thuringiensis and analysing the insecticidal potential of ...

12. Management for Scientists: Giving Feedback

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    ...regular, informal feedback is a very effective way to bring about change in behaviors and habits in a nonthreatening...

13. Plasmids 101: Antibiotic Resistance Genes

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    ...powder form, but quickly breakdown in solution. Storing aliquots at -20oC and avoiding repeated freeze/...

14. Antibodies 101: Epitope Tags

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    ...ideal with Myc, as low pH conditions are required during the purification, which may affect protein function...

15. Plasmids 101: TOPO Cloning

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    ...electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...

16. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    ...changes favored blue-shifting more than others. During this process, they identified mWasabi, a bright...

17. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    ...accelerate ongoing COVID-19 research. “I hope that by sharing the plasmids other scientists would be able to ...

18. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    ...This instability can cause partial loss of an ITR during routine plasmid propagation, with complete ITR ...

19. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    ...stable frameworks and structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672...

20. Plasmid Cloning by PCR

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    ...of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for cloning Run the PCR reaction...

21. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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    ...edition eBook include: new chapters on genome engineering, cloning, and plasmid tags, addtional content...

22. Another Pathway into Cells: iTOP

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    ... Mary Gearing...

23. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    ...differentiation.   Fig. 2: Single cell RNA-seq clustering of cells transduced with the TF pooled library...

24. Science Career Options

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    ...When preparing to graduate from university, many students are confronted with the question ‘what now?...

25. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    ... track the spatiotemporal dynamics of apoptosis during embryo development. GFP fluorescence increased ...

26. Visualizing Genomic Loci with CRISPR-Sirius

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    ...stability and minimize misfolding and recombination during virus production needed to deliver CRISPR-Sirius...

27. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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    ...thanks to Daniela Bourges for editing this post and sharing her technical expertise in persistent identifiers...

28. Early Career Researcher Toolbox: Free Tools for Science Writing

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    ... most often completed your habit is useful for figuring out when to schedule times to write.  Pomodoro...

29. Scientists Map the SARS-CoV-2-Human Interaction Network

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    ...all these reasons, the QCRG is delighted to be partnering with Addgene, who will be taking over the distribution...

30. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ...PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article contributed by...

31. "Build Your Plasmid": The Game - Play to Teach

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    ...biology, cell biology, microbiology, genetic engineering and strategic knowledge to solve a clinical disease...

32. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ...Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam...

33. Pushing the Limits of DNA Assembly

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    ...sgRNA for CRISPR experiments can be laborious, requiring multiple cloning steps. You can simplify this ...

34. 5 Great Apps for Lab Life

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    ...research in terms of preparedness, organization, and sharing data. Here are five FREE science lab apps that ...

35. Components of CRISPR/Cas9

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    ... endonuclease function, when used for genome engineering, the only CRISPR protein required is the Cas9...

36. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...here's a screenshot from the Chroma Spectra Viewer comparing a standard Cy3 or Rhodamine filter set (Chroma...

37. Antibody Guide

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    ...light orange, that bind to a specific antigen, triggering an immune response. The stem of the Y structure...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...protein:protein complex. Due to their increased manufacturing requirements, monoclonal antibodies are typically...tolerate freeze-thaw cycles. Here are a few tips for storing your antibody: Antibodies conjugated to fluorophores...a similar assay type) and search again. After filtering, narrow down further by company, price, and other...assay with the results of a non-antibody assay measuring either the same output or a proxy for that output... antibody validation This method validates by comparing your antibody of interest to a second, independent...

38. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ...because they are only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses...

39. Viral Vectors 101: Viral Applications

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    ...host cell (the target of viral infection) and delivering a genetic payload. This cargo is typically their...

40. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    ...brightness of two orange fluorescent proteins, differing by only a single amino acid. Without further information...