...performs independent research? Since launching the viral service in 2016 Addgene’s research team has been actively...believe that these results will be beneficial to the research community.    So, why are we so excited? For starters...inventory and more time providing new materials to the research community. References 1. Coroadinha, A. S., ...

...genome and proteins. This is, for the intrepid researcher looking for molecular biology tools, a very useful...replication When it comes to replication, viruses tend to march to the beat of their own drum. They have a wide ... eliminate or reduce the risk of infection — researchers were able to create far safer viral vectors that... The four types of viral vectors (which many researchers will informally refer to as viruses) are named...

...visibly complex - and unique - adult. My initial research directions followed that avenue with projects ...interests and skills than what fits for applied research. I focus in general terms on a couple of very ...teaching – often explaining a technical piece of research to others outside that specialty, who may be other...professional meetings, societies, networks just as do research scientists. There we meet others in the scientific...

...downward trend in funding and space in academic research labs has more students looking for other options...perspectives in science and engineering Looking at your research from a broader viewpoint may expose which experiments...extracurricular” may be taboo in the lab, multiple research studies highlight the importance of taking a break... skill sets Taking the time to identify and research 2 – 3 potential professions will highlight which...

...and flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, ...also includes clone names, which can allow you to search different providers for the same clone. This can...Antibodies community works to make best practices in research antibody selection and use possible, easy, and...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi....

...Finally, if you are a fellow researcher interested in adapting your research ideas into hands-on educational...students in functional genomics research Learn how the Arabidopsis research center brings plant science to...

...development by openly sharing their resources with the research community. To honor their legacy, we launched ...choice to share their fluorescent protein based research. This year, we put out another call for applications...genetic variants, what does that mean? Previous research shows that approximately 75% of all protein-coding...target of the anticoagulant drug warfarin. In past research, several VKOR variants have been found that affect...

...increasingly more cost effective and precise, researchers are now using it to investigate potential therapeutics...Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...correct disease hadn’t yet been realized. The researchers chose to explore its potential in mice with a...known as Crygc. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile...

...Addgene and we want to highlight it for use in your research. Martínez-García et al., BMC Microbiology 2011...progression. One of the major challenges in signaling research is the inability to replicate the spatio-temporal... post-translational modifications in cells and research into its many roles in protein function and signaling...at Yale University previously changed the way researchers can explore important questions surrounding serine...Nicola Patron's MoClo kit and her plant synbio research, read the Addgene interview. Engineering TALENs...order to extend the utility of the system, the researchers developed three new versions. Flipbow employs...

... Optogenetics Plasmids for Use in Neuroscience Research Neuroscience covers many subfields When I first...neuroscience and other fields. For instance I met one researcher studying how the microbiome can affect Alzheimer's...projects using biological tools. In addition to the researcher using sequencing technologies to study microbiome...making more AAVs and educational content to help researchers use these great tools. If you’re just getting...

...plasmids into lentiviral preps to speed up COVID-19 research even further. The following lentiviral preps are...lentiviral prep here Control AAV Control AAV allow researchers to not only interpret their results, but also...to achieve different patterns of expression, researchers often need to determine these transduction patterns... viral protocols and videos Find AAVs for your research ...

...screening technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using...have primarily used Cas9 to knock out genes, researchers at the Genetic Pertubation Platform at the Broad...screens because of its lowered editing rate. This research aimed to increase the potential of Cas12a genetic...

...method. Read on to learn how to use it in your research! The starting point: Ligation-independent cloning...only one type of dNTP in the reaction mix, the researcher can decide exactly how far the T4 exonuclease... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...assembly, and its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless...

...Drawing on current questions being asked in MIT’s research labs, BioBuilder inspires students and teachers...malleable curriculum, BioBuilder takes cutting-edge research projects in synthetic biology and transforms them...and watching animations that present a specific research problem and experiment to tackle. Next it’s off...and provides a nationally recognized platform in March to exhibit projects at any stage of completion, ...

...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...secondary antibodies would save a good deal of it. Researchers are continuously looking for ways to improve ...providing needed flexibility in assay development. Researchers who understand how and why to use secondary antibodies...

...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...commonly, antibodies of the IgG isotype are used for research purposes. Isolating polyclonal antibodies from...antibodies are particularly useful for certain research applications. Many secondary antibodies are polyclonal...Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...

...things. Now, work from Connie Cepko’s lab allow researchers to activate transcription or Cre recombinase ...activation The project began with a frustration many researchers have experienced: wanting to ask and answer experimental...not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to express a variety of ...and we’re eager to see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A...

...responsibility seriously. As I make my rounds speaking at research institutions and at scientific conferences, I ... is a style match for your needs can take some research.  It helps to spend some time thinking about what... Academic Labor Environment from the Future of Research Symposium Resources on the Addgene Website Check...Recent Job Openings Find Plasmids to Expedite Your Research   ...

..., expanding its practicality and popularity in research (Tanenbaum et al., 2014, Konermann et al., 2015...the most popular methods for CRISPR activation, research in the area has continued to develop. These newer...an engineered CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520...Addgene's CRISPR Guide Find CRISPR plasmids for your research ...

...required a one-hour presentation about a prior research project. In total, the interviews lasted from ...prepared with questions to ask. With a quick Google search for “questions to ask an interviewer,” you can ... they will ask you to present about your prior research. This is a chance to highlight your prior accomplishments...deciding to stop pursuing a particular line of research is a deliverable. Find 10 steps to a perfect science...