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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 443 results for: DUR

Showing: 421 - 440 of 443 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...Pluronic F-68 4475 µL Molecular Biology Grade Water Procedure Preparation Before handling any viruses, get materials...samples on ice or pre-chilled 96-well freezer blocks during use. In this protocol, a dilution series is prepared...

  2. Lentivirus ddPCR Titration

    Type
    Protocol
    ...15 mL DMEM Complete 3 µL of 250 U/µL benzonase Procedure Transducing Cells Thaw the virus on ice. Prepare...samples on ice or pre-chilled 96-well freezer blocks during use. To reduce the risk of contaminating reagents...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...institution-specific instructions. You must follow safety procedures and work in an environment (e.g. BL2+) suitable...HEK-293T cells that were inadvertently collected during harvesting. TIP: In lieu of centrifugation, you...

  4. DNA Quantification

    Type
    Protocol
    ...Restriction Digest of Plasmid DNA Background Information During several different stages of molecular cloning, ...

  6. Protocol - How to Streak a Plate

    Type
    Protocol
    ... (with appropriate antibiotic) Bacterial stab Procedure Obtain an LB agar plate with appropriate antibiotic...

  8. Water Bath Protocol

    Type
    Protocol
    ... bath weights and floats Reagents None needed Procedure Ensure that the water bath and water bath cover...

  9. Ligation Independent Cloning

    Type
    Protocol
    ...annealed but nicked vector product is then repaired during the replication cycle. Empty vectors for LIC typically...

  11. Gibson Assembly Protocol

    Type
    Protocol
    ...reaction. Can combine many DNA fragments at once. Procedure Design your plasmid and order primers (see figure...

  14. General Transfection

    Type
    Protocol
    ...the best ratio of mass DNA:mass PEI determined. Procedure Seed HEK293T cells at 3.8x10 6 cells per plate...

  16. Immunocytochemistry

    Type
    Protocol
    ...stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells Place a sterile poly-...

  18. Pipetting Protocol

    Type
    Protocol
    ...the P2 this represents the third decimal place. Procedure How to Pipette Now that you know the different...
Showing: 421 - 440 of 443 results