We narrowed to 445 results for: DUR

... following your institution’s hazardous waste procedures. Last Update: February 7, 2022 Protocol Video... filter. Aliquot and freeze upright at -20 °C Procedure Section 1: Affinity chromatography Equilibrate...

...for different cell types. Before starting this procedure, be sure that the cell pool has been sufficiently...well plate gave rise to 15–20 monoclonal lines. Procedure Generating Conditioned Medium (optional) Seed ...

...Poloxamer 188 4475 µL Molecular Biology Grade Water Procedure Preparation Before handling any viruses, get materials...samples on ice or pre-chilled 96-well freezer blocks during use. In this protocol, a dilution series is prepared...

...15 mL DMEM Complete 3 µL of 250 U/µL benzonase Procedure Transducing Cells Thaw the virus on ice. Prepare...samples on ice or pre-chilled 96-well freezer blocks during use. To reduce the risk of contaminating reagents...

...institution-specific instructions. You must follow safety procedures and work in an environment (e.g. BL2+) suitable...HEK-293T cells that were inadvertently collected during harvesting. TIP: In lieu of centrifugation, you...

...Restriction Digest of Plasmid DNA Background Information During several different stages of molecular cloning, ...

...learn how to create bacterial glycerol stocks. Procedure Follow the steps for Inoculating an Overnight ...

... (with appropriate antibiotic) Bacterial stab Procedure Obtain an LB agar plate with appropriate antibiotic...

...additional charge. What primers were used by Addgene during quality control? Addgene lists the primers used...

... bath weights and floats Reagents None needed Procedure Ensure that the water bath and water bath cover...

...annealed but nicked vector product is then repaired during the replication cycle. Empty vectors for LIC typically...

...the building blocks for many more complicated procedures. Name Description (Link opens in a new window...

...reaction. Can combine many DNA fragments at once. Procedure Design your plasmid and order primers (see figure...

...which may produce aerosols or splashes. These procedures should be done underneath a hood. Clean any spills...

...loading dye Electrophoresis buffer Pipet tips Procedure Select restriction enzymes to digest your plasmid...

...the best ratio of mass DNA:mass PEI determined. Procedure Seed HEK293T cells at 3.8x10 6 cells per plate...

...and prevents the vector from ligating to itself during the ligation process. If the sticky ends on either...

...stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells Place a sterile poly-...

...fit on a microscope stage) Reagents None needed Procedure Set up your microscope by removing any protective...

...the P2 this represents the third decimal place. Procedure How to Pipette Now that you know the different...