Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 993 results for: Des

Showing: 421 - 440 of 993 results

  1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...soCoChR-expressing system. Taken together, Shemesh et. al. describe a novel system for the precise mapping of neuronal...the single-cell level. Various soCoChR plasmids described in this work including the soCoChR-GFP plasmid...information about spatial and temporal organization. The design use to detect PKA activity can be generalized to...choice for FRET assays where a dark acceptor is desired due to its very low quantum efficiency. ShadowY...

  2. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...for affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman...towards solutions. The 2015 Boston FoR meeting was designed to explore areas where further data is necessary...for students interested in leading the process despite the recent advance. Similarly, grad students throughout...development and implementation of new strategies designed to cope with current increases. These two rulings...

  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of... of these options (2.7 kb with 24 nt guides), so it has the potential to eclipse any of the CRISPR nucleases...to examine Cpf1 off-target editing. First, they designed gRNAs with single and double mismatches throughout... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due its proximity to the cleavage...

  4. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...switches? FLEx (or “flip-excision”) switches were designed as a genetic tool for researchers to conditionally...does the FLEx switch work? Let’s say you want to design a genetic FLEx switch that turns BFP expression...single gene deleted in your knockout mouse, you can design a FLEx switch conditional knock-in cassette. As...experiment with a wildtype copy of a gene, you can also design FLEx switches to conditionally test the function...

  5. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...interest from its production site to its final destination. As an example, most secreted proteins are produced...pathway to their subcellular or extracellular destination. At the end of their life cycle, proteins may... about the subcellular structures your protein resides in and potentially which other proteins it forms...in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth review of quantitative colocalization...

  6. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...results can be affected by DNA quality and primer design, particularly for amplicons under 100 bp (Ma, 2015... concern, look for the lowest-cost option that provides you confidence in the results you need.  Whichever...by running the product on a gel. And only ddPCR provides absolute quantitative data, instead of relative...Polymerase Chain Reaction (PCR) Protocol: How to Design a primer  Molecular Biology Reference Molecular...

  7. PhD Applications After COVID

    Type
    Blog Post
    ...navigate a global pandemic can also be the thing that provides a new perspective that helps to clarify the harder...to introduce yourself to a few members of your desired department during the application process. Many...However, an online meeting with members of your desired program will allow for you to have the time and...during my application cycle, two schools on opposite sides of the country had scheduled visitation days during...

  8. Academic vs. Industry Postdocs

    Type
    Blog Post
    ... thanks to our guest blogger, Laura Desrochers! Laura Desrochers is currently a Postdoctoral Scientist...Apr 6, 2021. This post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca. Why...ve discovered.) As someone who has now seen both sides of the postdoc experience, I’m going to share what...

  9. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...die from cancer-related complications each year. Despite these grim statistics, new strategies for attacking...cancers, especially those of the pancreas and colon. Despite the prevalence of Ras mutations in a variety of... enabled researchers to begin discovering and designing molecules that are showing some promise in their...in the screenshot below). Currently, the page includes interactive maps for the Ras, mTor, and p53 pathways...

  10. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...researchers must co-transfect cells with 5’-P-ssDNA guides and an NgAgo expression plasmid to edit a gene ...Try NgAgo for Yourself On the other hand, the widespread success of CRISPR-Cas9 across multiple model ...vector or in vitro synthesis, which could be less desirable if one has to target multiple sites in a single...of the supposedly alien 5’-phosphorylated ssDNA guides, which might be rapidly degraded by the cellular...

  11. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...with various spacer lengths from 30-84 nucleotides, and designated this system REPAIRv1. Using next-generation...introduced through non-homologous end joining (NHEJ). Designing an RNA editor The Zhang lab envisioned a two-component...that interact with duplex RNA, they hoped to destabilize ADAR-RNA binding to decrease off-targets. Of ...

  12. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...are no exception to this effect -- meticulously designed experiments in labs around the world were put ...requests correlate with paper publication The dataset includes 11,426 plasmid requests over 6 years for 35 papers...into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential decay process we observed, while...model for each region using stochastic gradient descent in Pytorch, which gave us the maximum likelihood...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...that’s suitable for PCR amplification using pre-designed primers. By using this library to infect an animal...isolate DNA from the infected animal and use the pre-designed primers to determine which version(s) of the capsid... (figure 4f) of the tTA inducer virus, but the desired sparsity of labeling is achieved with lose doses...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology...

  14. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... on single papers could be more important.  Any slides, presentations, recordings, and other materials...those who were unable to attend. We capture the desktop and audio recordings of the meeting using teleconferencing... ensure that the journal club is useful for the widest audience, you should get feedback from those who...served. I hope that with some of the information described above you feel empowered to start your own journal...

  15. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...transfecting cells with a plasmid, such as PX459, which encodes Cas9 and contains a site for inserting a custom...targeted approach using RNPs has recently been described in vitro, with the hope of eventually using this...expertise. Final thoughts The Cas9-gRNA RNP method provides scientists with an additional means to deliver...other CRISPR delivery techniques Find tips on gRNA design Resources on Addgene.org Browse all CRISPR plasmids...

  16. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...whole field to a grinding stop and only now, 2 decades later, is it finally back in the limelight. On ...(such as viral vectors) without triggering a destructive inflammatory immune response. At the time, adenoviral...follow in the footsteps of Luxturna™. However, despite promising results in preclinical studies some potential...technology: engineering of new capsid variants, designing expression cassettes to regulate and optimize ...

  17. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...they wrote the job description. You check off all the boxes - you have the desired degree, you have mastered... gaps in scientists' transferrable skills. In describing the limitations of the study, the authors noted...over email. Examine your current and past job descriptions. Scan through all the things for which you are...

  18. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...those seen when using a plasmid backbone that was designed specifically for transient expression (find some...expression of viral constructs is a useful tool as it provides a means to quickly check that a construct is functional...3rd-generation transfer plasmids have a chimeric 5'LTR that includes a CMV or RSV promoter as well as a portion of ...those three genes plus tat. The envelope plasmid provides a heterologous envelope for pseudotyping and is...

  19. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. ...two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative...RNA editing methods — streamlining experimental design and its potential for clinical applications. Specifically...high-fidelity rate. Both Cas7-11 and PASTE open up multitudes of possibilities at the bench and potentially...

  20. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ... meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, Salmonella spp., Serratia...Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio vulgaris, Erwinia carotovora, E. chrysanthemi...meliloti, R. leguminosarum by. viciae, Rhodobacter sphaeroides, Salmonella typhimurium, Vibrio cholerae, Xanthomonas...
Showing: 421 - 440 of 993 results