We narrowed to 449 results for: ENA

...and how I felt about the different positions to enable them to decide for themselves.  Here is my experience...

...emphasize in order to be selected in foreign labs.” Elena Grosso (Mentee IMP 2014-15, University of Valencia...

...? What are your controls? Will these controls enable you to interpret your results? Will the experiment...

...should be made available for cell culturing and maintenance of the cell lines. Providing such an environment...

.... The Future What new types of research may be enabled by the use of AAVs? 1) Combined functional and ...

... the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence of Dox. In this...

...systematically test the potential causes of these phenomena by analyzing the plasmid and viral DNA sequences...

...: managerial duties ex: order materials, lab maintenance Administrative assistant: assists PI with admin...

...PMC3782611. 8. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling of off-target cleavage by...

... Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian...

....bios.2016.04.091 Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS, IMMUNOGENS...

...methanol, fix and permeabilize in a single step by denaturing proteins and dissolving lipids including those...

...mouse line itself. Generation, breeding, and maintenance of a mouse line is also an expensive and time-consuming...

...automation-friendly kits and/or automation systems that can enable you to run thousands of reactions with relatively...

...K. (2020). A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nature...

...protease inhibitors to lysate Insufficient denaturing conditions Adjust lysis conditions: time, temperature...

...Recombinant DNA Technology Chemistry techniques enable the isolation and purification of cellular components...recombinant DNA technology has overcome this obstacle by enabling the preparation of more manageable DNA fragments... enzymes, DNA ligases, and gel electrophoresis enabled the movement of specific fragments of DNA from ...which contains several restriction enzyme sites, enabling easy insertion of DNA by restriction enzyme digestion...are relatively small (∼3,000–6,000 base pairs) to enable easy manipulation. Typically, plasmids contain ...molecular cloning. The advent of molecular cloning enabled scientists to break chromosomes down to study their...

... on the same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic...family proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal...purification methods are more straightforward and enable direct identification of molecules associated with...editor Fusion of a Cas protein to a deaminase that enables direct base conversion in RNA or DNA without a ...2016). Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian ..., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., .... D., Gross, C. A., & Rosenberg, O. S. (2019). Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

...identity to be easily confirmed via sequencing, and enables the variable region and constant regions of the...given antigen. A mature B cell can clone itself to enable higher production of a single antibody, but each...blot Western blots use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel....additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run...primary antibody must be able to recognize the denatured configuration of the protein. For western blots...

...Cloning Chloroplast Toolbox Plant Expression Scott Lenaghan 122 plasmids with chloroplast-specific genetic...Dueber) for genetic engineering of S. cerevisiae , enabling larger multi-gene constructs, multiplexed CRISPR...GoldenBraid Cloning System and Toolkit Yeast Expression Elena Caro Bernat Plasmids for generating transcriptional...