We narrowed to 761 results for: Rest
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TypeBlog Post...added to the N or C terminus of your protein of interest. Many of these tags also have commercially available...makes it amenable to being left on your protein of interest, especially if downstream antibody detection is...
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Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...conditions without necessarily modifying the gene of interest in any way. For example, if green fluorescent ...situations. Tissue-specific genes – the genes of interest may not be expressed in the tissue/cell line you... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...activated in response to a ligand binding a GPCR of interest. How does TRUPATH work? The TRUPATH collection...transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2... -
Fostering communication in remote collaborations
TypeBlog Post...between recurring meetings, you should share interesting results or ask questions to your collaborators...microscopes and allowed me to investigate my protein of interest in a French clinical cohort, neither of which ... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...works to meet the individual needs of schools interested in using eScience Labs as part of their curriculum...Learn more about alternative science careers here Interested in creative science education tools? Check out... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under various... downstream of the open reading frame (ORF) of interest – try pRS plasmid shuttle vectors. You may prefer... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post...have in common? You can do quality science on interesting topics Having strong non-science skills is important...mentors, to discuss your strengths, weaknesses, interests and passions is another great way to shed light... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...getting researchers to the experiments they’re interested in faster by producing, titering, and testing...neuronal activity while studying your phenotype of interest. Optogenetic tools for similarly controlling neuronal... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...inducible CMV promoter and targeted to a sequence of interest with an sgRNA expressed from a separate plasmid...dCas9–APEX2 biotinylation at genomic elements by restricted spatial tagging), to confirm the composition ... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...blindness’ - the tendency for students to be more interested in studying animals than plants, and biology ...platform soon to be released. For individuals interested in ordering from ABRC for the first time, we ... -
What the HEK?
TypeBlog Post...with the viral plasmid containing the cargo of interest, and HEKs will produce and package viral particles...be a great option! Especially if your research interests include receptor biology, ion channels, or involve... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...scientists can use these to tag their proteins of interest. Antibody based probes, such as antigen-binding...epitope from the CDRs. To visualize proteins of interest tagged with the HA epitope, the lab fused a fluorescent... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog Post... had an alter ego as a mad scientist for show wrestling, founded a nonprofit, learned to play the ukelele...interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation builds... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...mice, by replacing the effector with a gene of interest in the cells you are studying. Read our SPARC ...single molecule-fluorescence assay to monitor restriction enzyme digestion of single molecules in real-... -
Meet the New Addgene Board Members!
TypeBlog Post... development was something Stegmeier has been interested in for a long time. “At an early age, I was fascinated...reagent, it’s an annotated reagent.” He’s also interested to see Addgene expand the sharing platform to... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...downstream applications (Berns, 1972). Using qPCR and restriction enzyme based methods, we showed that the DNA ...easily customized to detect other sequences of interest. While the VGS process isn’t new to Addgene,... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog Post...a graduate student at Kyoto University. He is interested in developing genetic tools for neuronal circuit...professor at Kyushu University. He has a particular interest in the development of sensory systems focusing... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...candidate with extra experience or a marketing interest that would be able to apply themselves immediately...immediately to the position. In case you are interested yourself, sales and marketing positions like these ... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog Post... Mentee: Make a list of topics/skills you are interested in exploring. Mentor: Make a list of topics/...smaller, actionable steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It ... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...optogenetics advance, the big questions that motivate his interest in neuroengineering, and some of the optogenetic...calcium or some other specific ion that would be interesting. It would also be great if you could make neural...