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...have in common? You can do quality science on interesting topics Having strong non-science skills is important...mentors, to discuss your strengths, weaknesses, interests and passions is another great way to shed light...

...inducible CMV promoter and targeted to a sequence of interest with an sgRNA expressed from a separate plasmid...dCas9–APEX2 biotinylation at genomic elements by restricted spatial tagging), to confirm the composition ...

...blindness’ - the tendency for students to be more interested in studying animals than plants, and biology ...platform soon to be released. For individuals interested in ordering from ABRC for the first time, we ...

... had an alter ego as a mad scientist for show wrestling, founded a nonprofit, learned to play the ukelele...interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation builds...

...mice, by replacing the effector with a gene of interest in the cells you are studying. Read our SPARC ...single molecule-fluorescence assay to monitor restriction enzyme digestion of single molecules in real-...

... development was something Stegmeier has been interested in for a long time. “At an early age, I was fascinated...reagent, it’s an annotated reagent.” He’s also interested to see Addgene expand the sharing platform to...

...downstream applications (Berns, 1972). Using qPCR and restriction enzyme based methods, we showed that the DNA ...easily customized to detect other sequences of interest.   While the VGS process isn’t new to Addgene,...

...display libraries In surface display, proteins of interest are fused to anchoring proteins to be expressed... few steps! To check whether a library you’re interested in is available as a viral prep, look under “...

...with the viral plasmid containing the cargo of interest, and HEKs will produce and package viral particles...be a great option! Especially if your research interests include receptor biology, ion channels, or involve...

...getting researchers to the experiments they’re interested in faster by producing, titering, and testing...neuronal activity while studying your phenotype of interest. Optogenetic tools for similarly controlling neuronal...

...candidate with extra experience or a marketing interest that would be able to apply themselves immediately...immediately to the position. In case you are interested yourself, sales and marketing positions like these ...

... Mentee: Make a list of topics/skills you are interested in exploring. Mentor:  Make a list of topics/...smaller, actionable steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It ...

...optogenetics advance, the big questions that motivate his interest in neuroengineering, and some of the optogenetic...calcium or some other specific ion that would be interesting. It would also be great if you could make neural...

...conditionally and reversibly degrade proteins of interest fused to a degron, a domain that induces degradation...This toolkit makes it easy to tag your protein of interest to create conditional human cell lines. Lastly...

... Professor in Microbiology, and her group are interested in understanding the mechanisms behind Mycobacterium... Julio Ernesto Valdivia Silva is a master of interesting combinations: biomedicine and astrobiology. During...

...than those without Addgene deposits? Even more interestingly, could we tell if depositing a plasmid with ...Sloan School of Management. She is particularly interested in exploring how innovative tools influence the...

...governed how this game was played. These rules restricted which cards could be played when and, depending...founder and consultant at FRESCI particularly interested in advanced in vitro models technology. Follow...

...understanding how, when, and where TFs work is of broad interest to the scientific community. Recently, the Zhang... The TF map is a valuable resource for anyone interested in cell differentiation programs. The Zhang lab...

...You can also enhance expression of your gene of interest with a variety of Cas9 activators available in...model that reflects the underlying biology you're interested in studying. You need to make sure your assay...

...RNA (gRNA) used to target the genomic locus of interest. As these off-target effects can be genome-wide...can allow for targeting of your genomic loci of interest that may not be accessible by wild-type Cas9, ...