We narrowed to 559 results for: SEC

...different lengths and sequences, as unintended secondary structures in your pegRNA could inhibit editing...

...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...

...luciferase, cre/lox, yeast expression, worm expression, insect expression, plant expression, mouse targeting, ...

...PMC5693259.  Wang, Han, et al. "Split cGAL, an intersectional strategy using a split intein for refined spatiotemporal...

...engineered mouse seems less overwhelming! In the second blog post of this series, I’ll discuss crossing...

...divide the viral elements among multiple plasmids. Second generation packaging systems use 3 plasmids; an...

... accommodate up to 8.2 kb of transgene DNA. The second generation vectors are deficient in E1, E2, E3,...

...these high levels of viral replication are toxic. Second, infecting subsequently higher and higher order...

...embryology techniques such as basic observation and dissections. Students don’t usually perform more modern techniques...

...behaviors are available from Addgene and other sources; second, characterizing a part or optimizing a TU is much...

...sequence, including propensity for off-targets, secondary structures, etc., as well as the presence of a...

...fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural...

...experiments performed will be useful in perpetuity.  Second, plasmids are easy to store and share, making recombinant...

... At least at Addgene, the questions in our FAQ section are really, truly frequently asked by customers...

...virtually any RNA sequence (Green et al, 2014). The second was an in vitro paper-based platform that allowed...

... to cleave again and possibly mediate HDR in a “second chance” mechanism. To prevent new editing post-...

...Laureates and the ever expanding importance of RNA The second keynote lecture was presented by Nobel Laureate...

...preparing the implant itself, which brings me to the second key point; the fiber optics need to be polished...

...as things came up. For example, I updated some sections of the NPA’s website. By volunteering, you can...

...things rolling. It can make or break the discussion section. And what about those audience members that want...