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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 494 results for: control

Showing: 421 - 440 of 494 results

  2. Fluorescence Titering Assay

    Type
    Protocol
    ... titer of your lentiviral vector allows you to control the multiplicity of infection (MOI) in downstream...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Inverted fluorescence...dilution with one well remaining as the untransduced control). Count the cells in the remaining well, a cell...

  3. Protocol - Bacterial Transformation

    Type
    Protocol
    ...should also add a positive control (many companies include a positive control plasmid with their competent...ligation of inserts to vectors than for an intact control plasmid. Incubate the competent cell/DNA mixture...

  4. Genetic Code Expansion

    Type
    Collection
    ...to bind to an unallocated codon. Therefore many controls must be used to make sure that these conditions...conditions are true. Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian...

  5. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...literature Highly charged amino acids Run control protein (if available) and note in analysis ...lysing protocol Not enough target Run protein control or load more lysate/sample* Underexposed ...

  6. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...insert. It is also important to set up negative controls in parallel. For instance, a ligation of the recipient...plasmid alone plate. The recipient plasmid alone control will tell you your “background” level or more specifically...see any colonies, you should conduct a positive control to ensure that your transformation worked. You ...depending on the number of background colonies on your control plate (the more background, the more colonies you...

  7. Immunocytochemistry

    Type
    Protocol
    ...Day 3-4: Fix and label cells Equipment Pipette controller Pipette tips and pipettes Rocking platform Tweezers...functioning as expected and specific, include a positive control sample that you know expresses the protein, such...express the protein of interest, and a negative control sample such as cells that do not express the protein...

  8. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...Multichannel pipette reagent reservoirs Pipette controller Microcentrifuge Microplate shaker ELISA plate... antigen Purified recombinant antibody Isotype control antibody HRP-conjugated isotype-specific secondary...mix. Dilute the primary antibody and an isotype control antibody to the desired concentrations in the antibody...recombinant purified Desmin (blue) or a negative control protein, Apolipoprotein L3 (red) overnight, blocked...

  11. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... a few cell types.  If you’re looking for more control in the cell types your lentiviral vector will infect...
Showing: 421 - 440 of 494 results