We narrowed to 535 results for: genome
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TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene...
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Synthetic Biology - Strains
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene... -
Bacterial Expression Systems
TypeCollection...Experiments Return to top Genome Engineering Modifying the bacterial genome by knocking out genes or introducing... CRISPR-based Genome Editing Systems For Editing in E. coli INTEGRATE: Bacterial Genome Engineering Using...controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like… Genetic Code Expansion... Protein Purification Resources The Structural Genomics Consortium (SGC) collection page contains an extensive...Bacterial CRISPR Plasmids page . Additional Addgene Genome Engineering Resources Learn more about CRISPR with... -
Synthetic Biology - Overview
TypeCollection...Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including...Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J.... -
Synthetic Biology - Metabolism
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...extremely accurate application, even in areas of the genome that are difficult to accurately read (Bell, 2018... -
Common Injection Routes in Mice
TypeBlog Post...Model Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...been viewed 219,289 times, while Validating Your Genome Edit has 76,011 views. Has all of this work helped... -
Plasmids 101: Colony PCR
TypeBlog Post...amplifies a product of the expected size, you know the genome of your bacteria already contains your target sequence... -
Synthetic Biology - Networks and Gene Regulation
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene... -
CRISPR Plasmids - Tagging
TypeCollection...Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...directly into this vector and targeted to the AAVS1 genomic safe harbor locus using untagged SpCas9 (Addgene...Zhang lab's PX330 , simultaneously targeting the genomic site and the donor vector. The PITCh donor plasmid...vectors to insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid...-programming sgRNA and HR donors for selectable genome modification. This system can be used to create... -
Zinc Finger Consortium Reagents
TypeCollection... these reagents are provided below: Zinc Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish...expressing zinc finger FokI nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...dCas9-FokI to dimerize at a given region of the genome. Like nickase technology, this technique reduces... -
CRISPR Plasmids - Drosophila
TypeCollection...: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and... -
Retrovirus Plasmids
TypeCollection...gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic proteins, ...This provirus then gets integrated into the host's genome by the accompanying integrase protein. When scientists...γ-retroviruses integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional... -
CRISPR Plasmids - Yeast
TypeCollection...Plasmids: Yeast Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ... -
Bioinformatics at Addgene
TypeBlog Post...had created a shared resource of pipelines for genomic data. Thanks to the flexibility inherent in Nextflow... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...(Alice Ting Lab 2016) Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015) Reference Feng... -
To Codon Optimize or Not: That is the Question
TypeBlog Post... that they can effectively inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org/10.1101/gr.231860.117...