... compared to controls without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal activation...

...Central PMCID: PMC4409680. 2. Mccullum, E. O., Williams, B. A., Zhang, J., & Chaput, J. C. (2010). “Random...

...fluorescent proteins that behave as monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen,...

...aptamers for imaging small non-coding RNAs in mammalian cells." Nature communications 9.1 (2018): 656....

... Design-Build-Test-Learn cycles. If you’re not familiar with this, it’s a method for building and testing...

...your protein of interest (POI). This approach is reliable, but it’s overkill in many cases. Often, you may...

...from your single cells, as duplicates can’t be reliably analyzed on their staining signals. For this, ...

... conformational complexity There are 24 known mammalian integrins, all heterodimers composed of one of...

...of Replication The Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101 Articles...

...for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression ...

...You may be familiar with immunofluorescence (IF, often referred to as immunocytochemistry (ICC) when ...

...including belittling opinions, public professional humiliation, accusations regarding lack of effort, intimidating...

...Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering...

...ligase, conditions and suitable probes, LDR can reliably detect single-base differences. The original creators...

...that steers the organization. As an Addgene vet familiar with the expertise and skills of many of my colleagues...

...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018...

...limit of detection of ~5%. This means they do not reliably detect editing events that occur in less that ...

...pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression...A206K) pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD...pCAG-DsRed2 - Mammalian Expression DsRed2-N1 - Mammalian Expression DsRed2-C1 - Mammalian Expression DsRed2...pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian Expression HcRed1-C1 - Mammalian Expression dKatushka...pQC mPlum IX - Mammalian Expression mPlum-N1 - Mammalian Expression mPlum-C1 - Mammalian Expression mPlum-pBAD... Sirius-N1 - Mammalian Expression SBFP2 380 446 13 Monomer (A206K) pSBFP2-C1 - Mammalian Expression Azurite...Monomer (A206K) mAzurite-N1 - Mammalian Expression mAzurite-C1 - Mammalian Expression EBFP2 383 448 17 ...

...gRNA_Cloning Vector 41824 Mammalian AfIII none S. pyogenes Bleocin Church MLM3636 43860 Mammalian BsmBI none S. pyogenes...pSPgRNA 47108 Mammalian BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none...sgExpression 48240 Mammalian BbsI yes, activate S. pyogenes Jaenisch pSQT1313 53370 Mammalian BsmBI none S....Gen) 48140 Mammalian BbsI yes, nick S. pyogenes EGFP Zhang PX460 (3rd Gen) 48873 Mammalian BbsI yes, nick... Gen) 48138 Mammalian BbsI yes, cut S. pyogenes EGFP Zhang PX335 (2nd Gen) 42335 Mammalian BbsI yes, nick...Gen) 42333 Mammalian BbsI yes, nick S. pyogenes Puro Zhang PX330 (2nd Gen) 42230 Mammalian BbsI yes, cut...42229 Mammalian BbsI yes, cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI ...

...cTnT Mammalian Campbell 89573 pCAG-iCre iCre CAG Mammalian Wong 89575 pCAG-VCre VCre CAG Mammalian Wong... CAG Mammalian Scholer 125574 pCAG-Cre Cre CAG Mammalian Imai 125577 pCAG-iCre iCre CAG Mammalian Imai...p209 pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green 8401 p224 pCMV-RFP...floxed RFP within CREM CMV Mammalian Green 8403 p153 pPr-cre Cre probasin Mammalian Green 8404 p236 pPr-CREM...CREM probasin Mammalian Green 8405 p240 pPr-CREM/Ins CREM with insulators probasin Mammalian Green 8406 ...separated by insulator probasin Mammalian Green 11543 pPGK-Cre-bpA Cre PGK Mammalian Rajewsky 11916 pBS185 CMV-Cre...CMV-Cre Cre hCMV Mammalian Sauer 11918 pBS513 EF1alpha-cre Cre EF-1 alpha Mammalian Sauer 11919 pBS448...