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Showing: 421 - 440 of 949 results

  2. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps...is to enhance Collections so that a group or organization can annotate their sequences from a shared database...sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered by SnapGene...

  3. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...done to and for us? BMC Biology, 15, 91. https://doi.org/10.1186/s12915-017-0433-z. PMID: 29052511. Louten...Replication. Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-800947-5.00004-1 Taylor, M. W...40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 More resources on the...

  4. What the HEK?

    Type
    Blog Post
    ... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

  5. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...fascinating biology is found in weird and rare organisms so I found it hard to stick to vertebrates alone...afield at first and I let them pass by. But I didn’t forget: what would it be like? Then I spotted an opening...Subscribe to Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...

  7. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan...al. Cell  (2015) can be found here: https://www.addgene.org/browse/article/9593/ Plasmids described in...al. Cell  (2013) can be found here: https://www.addgene.org/browse/article/6441/ Resources: An open source...

  8. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...American Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3.... Cleveland Clinic. Gram Stain. https://my.clevelandclinic.org/health/diagnostics/22612-gram-stain Hardy...

  9. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads and insufficient...processing mechanism, which is conserved in all living organisms and has previously been used for multiplex Cas9...nuclease editing, in virtually any cell type or organisms of interest with only minimal genetic payloads...

  10. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ... paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi.1006508 Vicens Q, Bourne PE...Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi.0030044 Wuchty S, Jones BF... of Knowledge. Science 316:1036–1039 . https://doi.org/10.1126/science.1136099  ...

  11. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...cleavage. Secondly, fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence...dynamics of apoptosis both in vitro and in living organisms to better study and understand these diseases ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

  12. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...extreme life on earth including extremophile microorganisms, biomarkers, and recently, cancer research ...Addgene has partnered with various community organizations and workforce projects to reach audiences beyond...Previous Awardee Kwabena Duedu Resources at Addgene.org Check out our CRISPR resources and CRISPR for...

  13. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ...identification of research reagents, several journals or organizations now recommend or require authors to use specific...well-established prefix resolution services such as Identifiers.org and N2T.net. To help authors comply with journal...Addgene_87155” and the following URL (https://scicrunch.org/resolver/RRID:Addgene_87155) will take you directly...

  14. Science Career Options

    Type
    Blog Post
    ...that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples...the Contacts for Your Dream Job Resources on Addgene.org Addgene Careers Page ...

  15. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...never forget the strawberries. Grand gestures are memorable! Attend events for one organization or go ...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...

  16. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...action plans! As a project progresses it is easy to forgot the original plan. Write down your plans in a shared...develop to reach their full potential in the organization. This will result in more engaged, successful...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete...

  17. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... using a viral approach Genetically modified organisms like transgenic mice are important tools for understanding...specific to interneurons in mice as well as other organisms, opening the possibility of using these tools ...protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1), some of the exciting... a stable monomeric form as determined by the organized smooth endoplasmic reticulum (OSER) assay. A major...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms.    Q11: How consistent is expression is when ...Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...

  19. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...stakeholders, the product managers collate and organize that information and form it into a roadmap--a... features, programs, or tools to build. After organizing the information, a software product manager works...everything running smoothly, you’ll want to be really organized. Often, product managers will have three or more...

  20. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Richter MF, Zhao KT, Eton E...compatibility and activity. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0453-z Additional resources ... in our CRISPR 101 blog series Resources on Addgene.org Find base editors for your research Read our...
Showing: 421 - 440 of 949 results