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Showing: 421 - 440 of 954 results
  1. Build Your CRISPR Vocabulary

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    Blog Post
    ..., 641–656. https://doi.org/10.1038/s41579-022-00739-4 More resources on addgene.org  Addgene's CRISPR ...distinguishing the foreign sequences and keeping the array organized. Target strand: The strand of foreign DNA that...
  2. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...Searching, Reading, & Organizing Write for the Addgene Blog Resources on Addgene.org Check out Our Revamped...straight to the point. 3. Engage the reader Scientists forget that the reader is not in their head. The scientist...
  3. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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    Blog Post
    ...genome-wide, and has been widely applied in many model organisms and cell lines. Although ChIP-seq is a relatively...cells. However, when working on specific tissues or organs in whole animal models like Drosophila melanogaster...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...
  4. Running for Rare Disease, Running for FOP, Running for AJ

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    Blog Post
    ... Disease that runs on behalf of the National Organization for Rare Disorders (NORD). NORD raises money...We will be collecting donations for IFOPA, an organization dedicated to raising funds and awareness of ...Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease...
  5. Addgene at the ASM Microbe 2016 Conference

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    Blog Post
    ...getting a badge illustrated with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth....with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...Network at Scientific Conferences Resources on Addgene.org Check out Our Microbiology Resource Page Deposit...
  6. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post
    ...fascinating biology is found in weird and rare organisms so I found it hard to stick to vertebrates alone...afield at first and I let them pass by. But I didn’t forget: what would it be like? Then I spotted an opening...Subscribe to Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...
  7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...done to and for us? BMC Biology, 15, 91. https://doi.org/10.1186/s12915-017-0433-z. PMID: 29052511. Louten...Replication. Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-800947-5.00004-1 Taylor, M. W...40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 More resources on the...
  8. What the HEK?

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    Blog Post
    ... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...
  9. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post
    ...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps...is to enhance Collections so that a group or organization can annotate their sequences from a shared database...sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered by SnapGene...
  10. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post
    ...Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan...al. Cell  (2015) can be found here: https://www.addgene.org/browse/article/9593/ Plasmids described in...al. Cell  (2013) can be found here: https://www.addgene.org/browse/article/6441/ Resources: An open source...
  11. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...cleavage. Secondly, fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence...dynamics of apoptosis both in vitro and in living organisms to better study and understand these diseases ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...
  12. Revamp Your Lab Meetings With Creative Virtual Collaboration

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    Blog Post
    ... paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi.1006508 Vicens Q, Bourne PE...Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi.0030044 Wuchty S, Jones BF... of Knowledge. Science 316:1036–1039 . https://doi.org/10.1126/science.1136099  ...
  13. Networking Follow-up: I Met a Person. Now What?

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    Blog Post
    ...never forget the strawberries. Grand gestures are memorable! Attend events for one organization or go ...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...
  14. Science Career Options

    Type
    Blog Post
    ...that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples...the Contacts for Your Dream Job Resources on Addgene.org Addgene Careers Page ...
  15. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...American Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3.... Cleveland Clinic. Gram Stain. https://my.clevelandclinic.org/health/diagnostics/22612-gram-stain Hardy...
  16. DAP arrays for multiplex gene editing

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    Blog Post
    ...multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads and insufficient...processing mechanism, which is conserved in all living organisms and has previously been used for multiplex Cas9...nuclease editing, in virtually any cell type or organisms of interest with only minimal genetic payloads...
  17. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...extreme life on earth including extremophile microorganisms, biomarkers, and recently, cancer research ...Addgene has partnered with various community organizations and workforce projects to reach audiences beyond...Previous Awardee Kwabena Duedu Resources at Addgene.org Check out our CRISPR resources and CRISPR for...
Showing: 421 - 440 of 954 results