We narrowed to 883 results for: prot

...resources: plasmid, pooled library, blog posts, protocols, and more. Selecting crispr in Guides will return...collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration in Parkinson’s disease...lentiviral prep needed Tags: Identify a specific marker protein Use: Similar to “Vector Type” you can filter by...about the subject, link to related guides and protocols, and provide easy to sort tables of hand-picked...

... tries to “optimize” a particular standard lab protocol. For example, someone may attempt super quick ...quick incubation times in a Western Blot protocol and then blame the antibody when no signals are obtained...so many things which can go wrong in a lengthy protocol. And customers may tell all sorts of details about...reviewing customer data sets, troubleshooting protocols, analyzing information, and solving customer problems...

...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue to dramatically increase...CRISPR applications. The first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

...producing vectors from plasmids — including the protocols we use in our lab — being able to order high quality...biosensors, optogenetic tools, and chemogenetic tools: protein tools that are activated, respectively, by substrates...educational resources, from eBooks to guides to protocols, which was why I was so excited to delve into ...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https...

...group box 1 protein. Normally localized to the nucleus, if the cell dies from injury, this protein leaks out... asbestos. However, as the U.S. Environmental Protection Agency has noted, there is no safe level of asbestos...thus ensuring that future generations will be protected from this dangerous material. This post was contributed...

...plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected...your cells. Regardless, the amount of reporter protein activity correlates to both the amount of DNA transfected...cells and the ability of the cells to express the protein.   In analysis of the result in Figure 1, an internal...were transfected successfully and expressing the protein. For example, fluorescence microscopy images resulting...

...overhang. This will serve as both a substrate for proteins required for strand invasion and a primer for ...preferred for large insertions such as fluorescent proteins or selection cassettes. This class of template...the CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013). PubMed. A Mouse ...alleles using long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (...

...hybridization for detection of gene copy number changes." Protocols for Nucleic Acid Analysis by Nonradioactive Probes...engineering transgenic constructs and mice." Current Protocols in Cell Biology (2009): 19-10. PubMed PMID: 19283728...fourth-generation DNA sequencing technology." Genomics, proteomics & bioinformatics 13.1 (2015): 4-16. PubMed PMID...by PCR Resources on Addgene.org Check out Our Protocol Pages Find Molecular Biology Tools Analyze Your...

...of indels and thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes...code for amino acids near the N’ terminus of the protein, in order to mitigate the ability of the cell to...for amino acids close to the C’ terminus of the protein, to maximize the chances of creating a non-functional...nucleotides, restricting gRNAs to 5 – 65% of the protein coding region will still result in many dozens ...

...series, you'll learn about new red fluorescent proteins, AAV tools for targeting the nervous system, and... monomeric, and cyan-excitable red fluorescent protein. Listen to Our Segment on mCyRFP1 Hot Plasmid 4... - a new, bright, and monmeric red fluorescent protein.  Listen to Our Segment on mScarlet Special thanks...

...Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope protein for producing lentiviral and MuLV retroviral particles...Didier Trono pVSV-G Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particles Akitsu Hotta...Hotta pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for more information Miguel...

...Encodes insert(s) A plasmid with one or more genes, protein coding sequences, or other elements inserted into...backbones often contain epitope tags or fusion proteins that are appended to new inserts through cloning...gene cloned into your plasmid, any tags or fusion proteins contained in the plasmid, any mutations, and the...scientists to best utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files...

...significantly cheaper. When using a homemade plasmid prep protocol, it cost less than $10 to make 1000 lanes worth...ladder? You can find the Tan Lab’s DNA ladder prep protocol here and the pPSU1 and the pPSU2 plasmids are ...Use the Kit-Free Alkaline Lysis Plasmid Miniprep Protocol Learn How to Analyze Your Restriction Digests ...

... new ways to learn about science careers, lab protocols, and, of course, plasmids. Today we’re trying ...Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use this kit ...strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer/producer...

... in using bioinformatic and proteomic tools to discover genes and proteins associated with pathogenesis... T7 polymerase, EcoRI, and various fluorescent proteins. Dr. Duedu’s plans for these plasmids have a heavy...

...from other nucleic acids (genomic DNA and RNA), proteins and chemical contaminants that have been used ...give scientists an indication of purity versus protein contaminants. The 260 and 230 nm ratios should ...for your research Brush up on molecular biology protocols using our video collection ...

...interested in our fluorescent protein background. Download the orange fluorescent protein background.         ... might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and other ...

...viral trasduction (see the Zhang Lab's Nature Protocols Paper for more information). V2.0 This designation...variants, as well as links to the Zhang Lab's Nature Protocols Publication describing them here. References ...engineering using the CRISPR-Cas9 system." Nature protocols 8.11 (2013): 2281-2308. PubMed PMID: 24157548....

...come up. An MTA can resolve those questions! MTAs protect you and your institution, and almost all universities...rights – and the rights of your institution – are protected while we handle all the paperwork. Record ...Addgene is internationally recognized as being protective of both the providing institution and the receiving...

...the water. It’s thought that the worm produces proteins which affect the grasshopper’s central nervous...system: infected grasshoppers expressed different proteins in their brains compared to uninfected grasshoppers...manipulation in a grasshopper harbouring hairworm: a proteomics approach." Proceedings of the Royal Society B...