We narrowed to 405 results for: pho;

1. Hot Plasmids: Fall 2024

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   ...-I3, while initially dimmer, showed greater photostability and also matured considerably faster, with ...the intestine. Figure 3: Expression of fluorophore-tagged GOLG-4 through eight stages of embryonic...

2. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   ...have diverse traits with good genetic mapping morphology and interesting behaviors. There is diversity...Chisholm) This cyanobacterium is the smallest photosynthetic cell in existence, but most abundant cell on...

3. Viral Vectors 101: Pseudotyping

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   ...protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density lipoprotein receptors (LDLRs...in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman...

4. Antibodies 101: Immunoprecipitation

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   ...sample lysates will also contain proteases and phosphatases that may degrade the target protein. To prevent...prevent this, include protease and phosphatase inhibitors in the lysis buffer, keep samples on ice, and ...

5. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ...this unique sequence of DNA and both identified lymphocyte antigen 6 complex (LY6A) as a receptor for PHP.B...discovered as an antigen upregulated on activated lymphocytes and is commonly used to enrich adult mouse hematopoietic...

6. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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   ...complement guide (see Figure 1). Perform standard phosphorylation and annealing of the two oligos. Figure...

7. An Integrin Antibody Toolkit from IPI

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   ...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...affinity reagents to study the difference in cell morphology changes induced by integrins binding the RGD ...

8. Evolution of Lab Techniques

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   ... at Harvard University. Just as computers, cell phones, and cars become more technologically advanced ...sequences among DNA fragments separated by gel electrophoresis." Journal of molecular biology98.3 (1975): ...

9. 27 Hot Plasmids from 2016

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   ...expression constructs fused with epitopes or fluorophores, generate expression vectors with various promoters...kinase is to express them with an appropriate phosphatase, a technique originally pioneered by Markus Seeliger...(Seeliger et. al. 2005). In this kit, Lambda phosphatase (Plasmid 79748) enhances bacterial expression...

10. 9 tips for a successful postdoctoral experience

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    .... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...