We narrowed to 732 results for: SON

1. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ...Y or fourth chromosomes, you are a very unlucky person. Animals are diploid except for their germ cells...

2. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy...

3. Polymerase Chain Reaction Overview and Applications

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   ...droplets is determined and then analyzed using Poisson statistics to determine the concentration of the...

4. Hot Plasmids: Winter 2025

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   ...plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song, Y. J., Nguyen, T. H., & Kim, J.-Y. (2024). Optimized...

5. Academic vs. Industry Postdocs

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   ...is not always the case. There are “difficult personalities” in every work setting and no manager will ever...

6. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...forward the next year when both the Yamanaka and Thomson labs showed the successful generation of human ...

7. Addgene's Cancer Collection Pages Connect the Research Community

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   ...Cancer – a term familiar to almost every person regardless of profession, race, or gender. Almost no ...

8. Plasmids 101: Optimizing Plasmid Yields

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   ...of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing low yields...

9. Genome-wide Screening Using CRISPR

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   ...Resources Page. References McDade JR, Waxmonsky NC, Swanson LE, Fan M (2016) Practical Considerations for Using...

10. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...accidental escape to a negligible level. One major reason we chose to publish the concept and likely capabilities...

11. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...talking to people and representing yourself as a real person, so that students see academics as well-rounded...

12. Tips for Using BLAST to Verify Plasmids

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    ... Jason Niehaus...

13. Which Fluorescent Protein Should I Use?

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    ...effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer...

14. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...Medical Center.  You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative...

15. CRISPR 101: RNA Editing with Cas13

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    ...off-target editing. In one direct transcriptome-wide comparison, REPAIRv2 reduced off-target sites from the 18,385...

16. AAVs in Retinal Gene Therapy

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    ...gene therapy revolution and the development of personalized medicine. References and further reading 1....

17. Getting to Know Expansion Microscopy

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    .../10.1126/science.1260088 Damstra HG, Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue...

18. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    ...lines in B-D represent the pure DNA spectra for comparison. Each of these contaminants will have a slightly...

19. Great Results Start with Great Standard Curves

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    ... ELISAs and When to Use Them A Control for All Seasons More resources on addgene.org Addgene's Antibody...

20. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where...

21. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...single flask. Through a bit of stochastic magic and Poisson statistics, each sub-population has, on average...

22. Plasmids 101: Colony PCR

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    ...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org ...

23. Tips for Screening with Yeast Two Hybrid Systems

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    ...43:729-736. PubMed PMID: 3907859. 4. Fields, S., Song, O. A novel genetic system to detect protein-protein...

24. Plasmids 101: Protein tags

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    ... Additional resources: Young CL, Britton ZT, Robinson AS, Biotechnology Journal 2012 , (7): 620-634....

25. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...boring. I think that such tendency is one of the reasons why we have only three biology-related Nobel prizes...

26. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ... among the earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study ...

27. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ...

28. Plasmids 101: Plasmid Incompatibility

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    ...plasmid. Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of ...

29. An Inside Look into Addgene's Dev Team

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    ... area, and when something urgent comes up, that person is the first in line to resolve it for expediency...

30. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...computed during generation of the ICE Score, the Pearson correlation coefficient (r) is also computed and...