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We narrowed to 1,076 results for: TOR;

Showing: 661 - 690 of 1076 results

  1. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ...Erin M Schuman, a former Caltech researcher, now Director at the Max Planck Institute for Brain Research...to all audiences. Right now, she is a science instructor at The Innovation Institute in Boston. When Eugenia...

  2. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...is correct. It's also best to use 1-2 ug of the vector for digestion. PCR Design primers that overlap ... probably left your plate for too long in the incubator. The small colonies are called satellite colonies...after the screening, sequence the plasmid. Many vectors contain sequences that are complementary to standard...

  3. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...of emitted photons before they are destroyed. Factors that affect photostability The photobleaching rate...fluorophore will bleach. This is, however, not the full story. The photobleaching rate does not depend on excitation... for each of these conditions. On top of these factors, environmental conditions such as cellular redox...

  4. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm...six figures. Averaging ten panels each. (For this story, it is important to know that the student presenting... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    ...way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly...of e-Amino Groups Available for Conjugation of Effector Molecules to Monoclonal Antibodies. Hybridoma ...resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies 101: The Basics of Western...

  6. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...team and software engineers to update our Lab Inventory Management System (LIMS) to accommodate our new... discussed them in meetings and 2) creating a pictorial guide for our engineers (Fig. 2). Creating this... a lot on context. New people in the lab or collaborators will not have the context you do, which means...

  7. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse protein ...GBPs together to create a complete transcription factor, activating luciferase transcription. Tang et al...delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is ...

  8. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...aligns with your work, your advisor may want to mentor you during the grant application process. When ...first. Additionally, reach out to peers and collaborators who have succeeded at grant writing to see what...write your first grant proposal" by Soapbox Science Editor in Nature Blogs "How to write a research project...

  9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some ...particular tool has gained widespread popularity – and notoriety – as a tool of choice in the field: dCas9 fused...Daniel M. Sapozhnikov is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working...

  10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...They play important roles in many cellular and regulatory processes, but much of their functionality is...effects can be minimized by disrupting lncRNA regulatory elements that are crucial for transcription and..., on the other hand, recruits transcriptional activators such as VP64, SAM, or SunTag to specific ncRNA...

  11. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...NG  - SpRY nuclease  C to T Base Editor SpRY A to G Base Editor SpRY   NYN/NRN NYN is less effective...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

  12. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...improved search algorithm which was trained on key factors from past PubMed user searches, such as clicks ...other areas of science. Scientists often post tweetorials, or threaded summaries, of their papers. These...publications in the fields of research they support. Monitoring these types of accounts can help you find papers...

  13. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ... practicing with your colleagues, ask them to brainstorm potential questions you may receive. People often...minutes per poster. Ask the presenter to give the “elevator pitch,” of his/her project – kindly let the presenter...Addgene website Become of a member of the Addgene depositor community Learn about careers at Addgene ...

  14. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...If you have ever worked in a molecular biology laboratory you have likely done a polymerase chain reaction...method that can help save time and effort in the laboratory.  There are two main categories of multiplex ... Variations Quantifying low abundance targets Monitoring subtle changes in target DNA levels Multiplex...

  15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...2019; Chen & Liu, 2023). This method uses a prime editor (typically a Cas9 H840A nickase fused to a reverse...optimization efforts. This holds true for any prime editor experimentalist, and especially shines once large-scale...Collection The CRISPR Guide Empty gRNA Expression Vectors...

  16. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your...maximize cell density and plasmid yield as these factors are generally, but not perfectly, correlated. ...manufacturer. On Addgene plasmid pages, you may see depositor recommendations to grow a plasmid at 30℃ or room...

  17. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene within the genome...gRNAs are then cloned into lentiviral transfer vectors to create the CRISPR library. CRISPR libraries ...library that is right for you There are several factors to consider when selecting a CRISPR library for...

  18. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...the sequencing results of the plasmids in our repository with known reference sequences, such as full ...full plasmid sequences provided by the laboratories that deposit their plasmids with us or other entries ...entries in NCBI’s numerous databases. As our repository has grown over the years (we now have over 60,000 plasmids...

  19. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Protein (GFP), a protein from the jellyfish Aequorea Victoria. Since that discovery, the family of FPs just ... used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato...have a filter that will pass blue light to the detector/camera, then BFPs are of no use to you. When using...

  20. MXS Chaining

    Type
    Blog Post
    ... construct. This strategy can be used to build vectors with diverse functions, and thus, is used in many...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...Shetty RP, Endy D, Knight TF. Engineering BioBrick vectors from BioBrick parts. Journal of biological engineering...

  21. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...Further complicating the issue is the long and ugly history of reporting false essentialist links between biology...actively look for and consider bias as a confounding factor when interpreting findings, particularly when researching...

  22. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...colonies. The best way to do this is by using your vector with and without an insert to verify that the primers...cultures of your positive clones. If you want to store your clones longer term, just streak the colonies...PCR primers work in the first place: the backbone vector with and without an insert. These controls are ...

  23. Plasmids 101: Protein tags

    Type
    Blog Post
    ...number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be...purification to get your protein as pure as possible? Vectors that offer different combinations of tags are readily...Protein (GFP) Fluorescent Protein Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his...

  24. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...applications? Fujii: We generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral...joined Addgene's Advisory Board, as well as the Editorial Board of Scientific Reports and PLoS One. Additionally...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for...

  25. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ... delivered as a pooled library via a lentiviral vector to cell lines that either stably expressed Cas9... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...hypersensitivity, or are in an enhancer, transcription factor binding site, or a coding region. Targeting these...

  26. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...each enzyme. In addition, Csm6, a CRISPR type-III effector nuclease, can be used in conjunction with Cas13...single-component programmable RNA-guided RNA-targeting CRISPR effector. Science 353:aaf5573 . https://doi.org/10.1126...

  27. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Western blot. When choosing beads, there are several factors to consider. Agarose beads are sponge-like and ...more stringent buffers may be needed. Since many factors, including salt concentration, ionic strength and...prevent this, include protease and phosphatase inhibitors in the lysis buffer, keep samples on ice, and...

  28. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... (LshCas13a) to begin to characterize this new effector (Abudayyeh et al., 2016). In the endogenous CRISPR...fluorophore. The fluorescent signal can be used as an indicator to determine whether the target sequence is present...single-component programmable RNA-guided RNA-targeting CRISPR effector." Science 353(6299) (2016):aaf5573. PubMed PMID...

  29. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers, aptabeacons (or aptatags...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence. Though ...

  30. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...logistical, like briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based...in the possibility of joining your team as a postdoctoral researcher. Dear Hiring Manager, I am writing...your cover letter, and if possible, send it to a mentor or colleague for a second look and helpful feedback...
Showing: 661 - 690 of 1076 results