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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 921 results for: Tro;

Showing: 661 - 690 of 921 results

  4. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems added another ... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due its proximity to the cleavage...

  6. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...ll need to produce your viruses in-house.   Introducing the systems There are many ways that viruses ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...

  7. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...protein’s structure into experimentally determined electron density, have been known to say something silly...educators. Using these hands-on tools, educators introduce their students to this otherwise invisible molecular...

  8. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...used to tag endogenous genes and simultaneously introduce a selection marker for screening modified strains...with a Cas9 expression vector. They can also be introduced directly into a Drosophila S2 cell line stably...

  10. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...not that I would know about this… A no template control (NTC) should always be included. While the physical...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...image acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...use of viruses allows much better spatiotemporal control over labeling methods as compared to transgenic...

  15. DNA Purification Without a Kit

    Type
    Blog Post
    ...but the addition of a high concentration of a chaotropic salt (guanidine hydrochloride for the plasmid... glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two...

  17. Tips for arabidopsis transformation

    Type
    Blog Post
    ...like to isolate a minimum of five. You have no control over how many insertions occurred. Having too many...segregate 3:1 in the T2 generation. You also have no control over where your construct will be inserted. If ...

  19. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems has added another... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the cleavage...

  22. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...defective chloramphenicol resistance gene (CamR) by introducing a point mutation (H193Y). The reversion of this...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

  23. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...that are more career-focused. After these talks, introduce yourself to the speaker and offer your business...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

  24. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...measurements. Extra care should be taken so as not to destroy the donor molecule. Fluorescence lifetime imaging...

  25. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...alongside standard surface labels. Here, I will introduce you to a few useful labeling and staining techniques...Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

  26. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...If you read my eBook and it helps you, please introduce yourself to me by email or in person. If you feel...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

  27. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...evident that widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR... need for thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the...

  28. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...Like graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist...women), visibility (how to get it, especially if introverted ), and networking (how to get people to remember...

  30. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...constructs were designed to be packaged into a retrovirus for infection of host cells. The fluorescent ... is composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase...
Showing: 661 - 690 of 921 results