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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,029 results for: ELL

Showing: 881 - 920 of 1029 results

  1. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...mixture of two base editor enzymes in mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR...bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity ...

  2. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ... brain regions. You can partially control which cells are successfully infected by an AAV by altering ...control where your gene of interest is expressed.  A cell-type specific Cre can provide you with that control...

  3. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...pathways that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis...pathway. “The idea is that if you introduce them into cells one at a time, you could identify those that confer...

  4. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn at UCSF have recently developed...

  6. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered ...demonstrating that CasX is active in both E. coli and human cells. Using the structure of CasX at eight different ...

  8. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ..., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...

  14. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ... region of a different species and/or isotype (Liddell 2013). In most cases, the resulting chimeric antibody...antibodies! References and resources References Liddell, E. (2013). Antibodies. In The Immunoassay Handbook...

  16. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...industry?” Once academia was the clear answer with a well-defined path to professorship. But recently a downward... professions increases your chances of finding a well-suited job. EAs shouldn't be viewed as an unproductive...

  17. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...again or set up a meeting. If you really got along well, suggest a coffee chat in the near future. Even ...you succeed in any way. Don’t believe people who tell you that thank you isn’t necessary. I once got a...

  18. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...skip purification steps and end-repair steps with well-designed fragment overlap sections, while still ...of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires upfront...

  19. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the... 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein...

  20. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Addgene deposits? Even more interestingly, could we tell if depositing a plasmid with Addgene causes a paper... most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry...

  21. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https...

  22. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...hard-to-read sentences.  Grammarly Grammarly is a lot like spell check, but since it’s an internet browser plug-in...managers and productivity tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management...

  23. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction...fantasy, pop-sci, true crime, and so on. We’ve read well-known classics, like Austen’s Pride and Prejudice...

  24. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...for the next 5 years. Does an academic career work well with your life and career plan? This might be a ... - includes videos on general resume writing, as well as additional videos with emphasis for graduate ...

  25. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...professors how they hire scientists for their labs. Tom Ellis selects new hires from a pool of CVs collected from... awhile. Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

  26. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... facet of a project this big. Maintaining our excellent service and accuracy involves caring and careful... it took a lot of work and dedication to do this well ... because it did. So finally, a big thank you ...

  27. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced...

  28. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...requirements in regard to their sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or...References References Adler, B., Trinidad, M., Bellieny-Rabelo, D., et al. (2023). CasPEDIA Database: ...

  29. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by...researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR...

  30. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...methylation should be considered when digesting DNA. Why? Well, even though Dam methylation sites are not specifically...later be verified by Sanger sequencing. How can I tell if my enzyme will cut? Whether performing a digest...

  31. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...are trying to block but your primary antibody as well.  Next, Fab and Fab’ fragments are almost interchangeable...fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016...

  32. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ... a journal club to my molecular biology class. A Cell paper. With six figures. Averaging ten panels each...strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes if I need...

  33. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence...convince the reviewers that the proposal is strong, well-thought out, and will be successful when funded....

  34. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...for my interviews in industry. Prepare stories to tell in your interview The STAR interview method is a...prepare stories to answer interview questions. Story telling shares many of the same elements as a STAR answer...

  35. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...contained all the goldilocks antibodies in one place... well, lucky for you one is in the making by Dr. Zhuhao... Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the Wu lab will be screening over...

  36. A Look at Addgene's QC Process

    Type
    Blog Post
    ... the deposit, NCBI sequence and protein data, as well as available backbone sequences. All of these resources..., with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary. Resources ...

  37. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...developed by the Allen Institute for Artificial Intelligence. Semantic Scholar analyzes publications and ...paper has is displayed at the top of the page, as well as the number of citations highly influenced by ...

  39. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    .... The computer scientists can get involved in modelling, the electronics folks can build a robot worm ...rich and complex microbiome data for analysis. And, well, cats... everyone knows the internet loves cats!...
Showing: 881 - 920 of 1029 results