Addgene

Tips to Make the Most of a Scientific Conference

Type

Blog Post

          ...professional network
Volunteer at a professional organization to expand your skill set

Resources on the Addgene...
        

Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Type

Blog Post

          ...libraries
Read all CRISPR blog posts

Resources on Addgene.org

Browse our CRISPR interference plasmids
Find...
        

CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Type

Blog Post

          ...gRNAs
Find other CRISPR software

Resources on Addgene.org

Find CRISPR plasmids and resources
Download ...
        

Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

          ...Download the Plasmids 101 eBook

Resources on Addgene.org0

Check out protocols for plasmid cloning
Find...

xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

          ...about Homology Directed Repair

Resources on Addgene.org

Check out our CRISPR Guide
Find CRISPR Plasmids...

Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

          ...: A Practical Guide

Additional Resources on Addgene.org

Check Out Our CRISPR Guide Pages
Find Empty ...

Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

          ...Addgene. The CRISPR Journal, 6(6), 493–501. https://doi.org/10.1089/crispr.2023.0059.
More resources on the...
        

Viral Vectors 101: Voltage Indicators

Type

Blog Post

          ...doi: 10.1038/s41598-019-52370-8
Resources on Addgene.org

Addgene’s Viral Services
Addgene’s Virus Protocols...
        

Antibodies 101: Conventional vs Spectral Flow Cytometry

Type

Blog Post

          ...Buffers, Storage, and Conjugates
Resources on Addgene.org
Addgene's Antibody GuideAddgene's collection ...
        

Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

          ... Used to Turn Genes On and Off

Resources on Addgene.org

Find Mammalian Expression Vectors
Find Cre-Lox...

Polymerase Chain Reaction Overview and Applications

Type

Blog Post

          ...Site Directed Mutagenesis by PCR

Resources on Addgene.org

Protocol: Polymerase Chain Reaction (PCR)
Protocol...

Academic vs. Industry Postdocs

Type

Blog Post

          ...Kamen's 8 tactics for choosing a lab

Resources on Addgene.org

Find open positions at Addgene
Check out our...

Tips for a 1st time AAV user (by a Rookie AAV user)

Type

Blog Post

          ...tips for a first time CRISPR user

Resources on Addgene.org

Learn how Addgene produces AAV and lentivirus...

Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

Type

Blog Post

          ...other plasmid cloning techniques

Resources on Addgene.org

Find basic molecular biology protocols
Find ...

Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

          ...Plasmids 101: Mammalian Vectors

Resources on Addgene.org

Check Out Our Protocols Pages
Browse Fluorescent...

Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

          ...Lab E. coli Strains

Additional Resources on Addgene.org

Find Info on How to Quantify Your DNA
Learn ...

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

          ...User (by a 1st Time CRISPR User)

Resources on Addgene.org

Browse CRISPR Plasmids for Mammalian Systems...

Genome-wide Screening Using CRISPR

Type

Blog Post

          ... Protocols in Molecular Biology 115: . https://doi.org/10.1002/cpmb.8
Additional Resources on the Addgene...
        

Droplet Digital PCR for AAV Quantitation

Type

Blog Post

          ...of our viral vectors blog posts

Resources on Addgene.org

Learn our protocol for AAV titration by qPCR...

SciComm: Taking Science to Elementary and Middle Schools

Type

Blog Post

          ...science communication blog posts

Resources on Addgene.org

Check out our comprehensive CRISPR guide
Find...

Isolating Genomic Regions of Interest with the CRISPR System

Type

Blog Post

          ...posts
Read our CRISPR cheat sheet

Resources on Addgene.org

Read our CRISPR guide
Find CRISPR plasmids by...
        

CRISPR 101: RNA Editing with Cas13

Type

Blog Post

          ... about CRISPR DNA Base Editing

Resources on Addgene.org

Browse All CRISPR Plasmids
Check out our CRISPR...

The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Type

Blog Post

          ...plasmid sharing during COVID-19

Resources on Addgene.org

Find plasmids from David Liu's lab
Visit our...

CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

          ...techniques
Find tips on gRNA design

Resources on Addgene.org

Browse all CRISPR plasmids
Find validated gRNAs...
        

Plasmids 101: Secondary Nanobody Toolbox

Type

Blog Post

          ... protein expression

Additional Resources on Addgene.org

Find more nanobody expression plasmids here
...

CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

          ... CRISPR Editing in Human Cells

Resources on Addgene.org

Browse All CRISPR Plasmids
Find Validated gRNAs...

Great Results Start with Great Standard Curves

Type

Blog Post

          ...
A Control for All Seasons
More resources on addgene.org
Addgene's Antibody Guide
Addgene's Ready-to-Use...
        

Simplify Cloning with in vivo Assembly

Type

Blog Post

          ... blog posts on plasmid cloning

Resources on Addgene.org

Read our protocols for plasmid cloning
Find ...

New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Type

Blog Post

          ...genome-wide screening using CRISPR

Resources on Addgene.org

Find CRISPR plasmids and resources here
Find...

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

          ...Methods
Validate Your Genome Edit

Resources on Addgene.org

Find CRISPR Plasmids for Your Research
Check...
        

Lentiviral Vector Uses and Overview

Type

Blog Post

          ...Screening Using CRISPR/Cas9

Additional Resources on Addgene.org

Check out Our Lentivirus Guide
Learn about Virus...

Plasmids 101: Colony PCR

Type

Blog Post

          ...about Gibson Assembly

Additional Resources on Addgene.org

Read about Choosing a Molecular Cloning Technique...

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

          ...Contribute to the Addgene Blog

Resources on Addgene.org

Browse Our Viral Vector Plasmids
Read Our Lentiviral...

Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

          ...just starting a lab, or are looking for a way to organize samples within your lab, we highly recommend using...
        

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

          ...directly from A. Victoria and expressed in any organism while still maintaining fluorescence.
The protein...
        

An Inside Look into Addgene's Dev Team

Type

Blog Post

          ...engine update
Find all Addgene news

Resources on Addgene.org

Check out our job postings
Learn more about ...
        

Deciphering the Mysteries of Behavior with Viral Vectors

Type

Blog Post

          ...Beginner's Guide to Viral Vectors

Resources on Addgene.org

Learn about Addgene's viral service
Find viral...

Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Type

Blog Post

          ...for gene expression in mammals.

Resources on Addgene.org

Search for AAV vectors to use with your research...

New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

          ...sheet
Download our CRISPR eBook

Resources on Addgene.org

Check out our comprehensive CRISPR guide
Find...
        

Supporting Reproducibility with a Connected ELN

Type

Blog Post

          ...posts about scientific sharing

Resources on Addgene.org

Find protocols of molecular biology techniques...

We narrowed to 965 results for: org