We narrowed to 464 results for: LARGE

1. PITChing MMEJ as an Alternative Route for Gene Editing

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   Blog Post

   ...whole-plasmid integration if you’d like to integrate a larger amount of material into the genome. Open questions...

2. Tips for Using BLAST to Verify Plasmids

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   Blog Post

   ...protein sequences (nr)” database as it contains the largest number of protein sequences. Just below the BLAST...

3. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   Blog Post

   ...are now available exist within the context of the larger bioeconomy, and that it’s been 40 years since the...

4. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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   Blog Post

   ...important regulator of embryonic development The largest conference room at Addgene is named Sonic Hedgehog...

5. Deciphering the Mysteries of Behavior with Viral Vectors

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   Blog Post

   .... However, most lesioning methods destroy areas larger than intended and may unintentionally remove connecting...

6. Using Video to Share Your Science: We Share Science

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   Blog Post

   ...abstracts identified. The journal Cell had the largest average view count per video abstract at 2,947 ...

7. The Challenges of Cell Culture

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   Blog Post

   ... match their original description. The oldest, largest, and most diverse of these repositories is the ...

8. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   ... can persist undetected; unlike infections with larger microbes such as yeast, fungi, or bacteria, mycoplasma...

9. Antibodies 101: Introduction to Immunofluorescence

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   Blog Post

   ...secondary antibodies on hand to mix and match with a larger collection of primary antibodies. This strategy...

10. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post

    ...generating small mutations with HDR, when it comes to larger scale genome editing, such as replacement of a ...

11. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post

    ... etc. Contamination by humans accounts for the largest source among those mentioned above. The contaminants...

12. Antibodies 101: Introduction to Antibodies

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    Blog Post

    ...(Cruse et al., 2004). Because proteins are much larger than this, it may contain many epitopes that antibodies...

13. Deep Dive: qPCR

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    Blog Post

    ...be worth the initial effort of setting it up for larger or frequently analyzed sample types and sets.   ...

14. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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    Blog Post

    ...modifications, efficiency can be low, especially for larger modifications. What’s the solution to this inefficiency...

15. Protocol - How to Purify DNA from an Agarose Gel

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    Protocol

    ..., and a lower percentage gel will help separate larger bands. 10% Rule For each sample you want to load...

16. Weighing Reagents Protocol

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    Protocol

    ...places. Pro-Tip If you’re weighing out an amount larger than the capacity of your balance or weighing boat...

17. Protocol - How to Ligate Plasmid DNA

    Type

    Protocol

    ...proportionally. 1μL of ligase should be sufficient for larger ligation reactions. Because ligase buffer contains...

18. Protocol - How to Run an Agarose Gel

    Type

    Protocol

    ..., and a lower percentage gel will help separate larger bands. 10% Rule For each sample you want to load...

19. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type

    Protocol

    ... of bacteria to grow (low copy plasmids require larger cultures). Centrifuge the culture to pellet the...

20. Virus Protocol - Generating Stable Cell Lines

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    Protocol

    ... individual wells become confluent, expand into larger vessels. A confluent well of a 6-well dish can ...