We narrowed to 761 results for: Rest
-
TypeBlog Post...governed how this game was played. These rules restricted which cards could be played when and, depending...founder and consultant at FRESCI particularly interested in advanced in vitro models technology. Follow...
-
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...conditionally and reversibly degrade proteins of interest fused to a degron, a domain that induces degradation...This toolkit makes it easy to tag your protein of interest to create conditional human cell lines. Lastly... -
Addgene Depositors Get More Citations
TypeBlog Post...than those without Addgene deposits? Even more interestingly, could we tell if depositing a plasmid with ...Sloan School of Management. She is particularly interested in exploring how innovative tools influence the... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...understanding how, when, and where TFs work is of broad interest to the scientific community. Recently, the Zhang... The TF map is a valuable resource for anyone interested in cell differentiation programs. The Zhang lab... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post... Professor in Microbiology, and her group are interested in understanding the mechanisms behind Mycobacterium... Julio Ernesto Valdivia Silva is a master of interesting combinations: biomedicine and astrobiology. During... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...You can also enhance expression of your gene of interest with a variety of Cas9 activators available in...model that reflects the underlying biology you're interested in studying. You need to make sure your assay... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...RNA (gRNA) used to target the genomic locus of interest. As these off-target effects can be genome-wide...can allow for targeting of your genomic loci of interest that may not be accessible by wild-type Cas9, ... -
A Deep Dive into BioBuilder
TypeBlog Post...partnership with high school teachers to boost interest and understanding around the engineering of biology...by emailing [email protected]. Of particular interest this fall: The BioBuilderClub BioBuilderClub ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...open-reading frame and separated from the protein of interest by a 2A self-cleaving peptide (Goedhart et al,...correlation between the fluorescent protein of interest and the reference protein (Goedhart et al, 2011... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...about the various posters I visited. If you’re interested in neuroscience but are afraid you don’t have...virus for you. Neuroscientists are particularly interested in using AAVs because they provide a highly versatile... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...and then unfollow those who do not capture your interest during that week.” You can also find Dr. Knoepfler...consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve: 1) convinced... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post...the intent is to make a knockout in your gene of interest, as it is prone to generating indel errors. Indel...bloggers! David Wyatt is a graduate student interested in determining how the structure of broken ends... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...along with an antibody targeting the protein of interest led to rapid, targeted degradation that is dependent...independently control expression of your genes of interest. These transcription factors consist of the GAL4... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post... approach to simultaneously clone two genes-of-interest (GOI) into two independent expression cassettes...pUC57-L3L2) that offer the advantage of using restriction digest cloning to introduce the GOIs into the... -
Human Germline Editing Using CRISPR
TypeBlog Post...s start with the paper itself: Ma et al. were interested in correcting an autosomal dominant mutation ... vs repair from the maternal MYBPC3 allele. Interestingly, they found that the embryos underwent repair... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...growth factors or serum to stimulate a pathway of interest, but there can be a lot of static since other ...inserting pdDronpa domains into loops of a protein of interest. If you plan to try this, it’s important to check... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...assay with your chosen FP in your cell type of interest before starting your experiments. Selecting a...this fluorescent protein to tag your protein of interest will be problem-free. In figure 1 we provide an... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...every project, time, attention, and/or length restraints mean you will have to pick and choose what concepts...feedback in a variety of ways, like having them restate or summarize the concepts you’re covering. Don'... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post... allows synthetic biologists to use Type IIS restriction sites to easily assemble multiple DNA fragments...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no...