We narrowed to 763 results for: SCR

...the results of studies on reducing antibiotic prescriptions for respiratory tract infections. The video ...provide more than enough time for researchers to describe their science, and for viewers to decide if reading...

...calendar invite over to the r/Science team with a description of our AMA and Joanne’s preferred time to start...receive via email. The cool features are too many to describe here, but I have over 2,000 notes which Evernote...

... lines you are using, it is best to start from scratch. Obtain a vial of the desired cell line from a ...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...

...positive control, the internal control vector, was described earlier. Once you are sure your conditions are...come from multiple, independent sources. We'll describe these a bit more below, but the papers cited in...

...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

...example, search "Cdc42", "transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...validation can be done using methods such as transcriptomics or proteomics. This approach is often appropriate...

... from a gene synthesis company such as IDT or Genscript, and cloning them sequentially into the chosen...combining the AAV and CRISPR-based approaches is described in several recent reviews (Howes and Schofield...

...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...unicellular organisms, they can also be used to easily screen multiple therapeutic compounds in one experiment...

...frames downstream of the gRNA target site so that transcripts undergo nonstop decay (Frischmeyer et al., 2002... mRNA Surveillance Mechanism That Eliminates Transcripts Lacking Termination Codons. Science 295:2258–...

...science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive) conflict...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

...) to remove mutated exons from the dystrophin transcript. Unfortunately, the oligonucleotides only modestly...in one experiment, they found the exon-skipped transcript constituted 59% of total dystrophin mRNA, similar...

...”, “colony”, etc. This will help save time by screening out irrelevant candidates, and, more importantly...importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools, skills...

...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

...reporter to show that the mutants abolished transcriptional activity, even in the presence of endogenous...carried by extracellular vesicles (EVs), edited transcripts could endow neighboring cells with pro-oncogenic...

...2025).In this latest work, the team designed and screened a large library of iGluSnFR3 mutants, identifying... insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to...

... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights...that are amenable to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox... Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression in organisms...

... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...and can immediately undergo replication and transcription. However, with scAAV the effective genome size...

...the opsin into the correct orientation to be transcribed (Figure 2) (Sharma and Zhu, 2014). How does FLEx...simultaneously recorded the electrical impulses of discrete subpopulations of this brain area, demonstrating...

... appealing to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were... which is a fusion between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called...