We narrowed to 869 results for: SOM

... down the hall, it is always a good idea to take some time to confirm that you are working with the correct...strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI) are capable of...lanes look like your uncut lane then there is something wrong! Quantify your DNA. Loading too much DNA...

...used working definition of systemic capsids. After some consideration, we offer this definition: A systemic...systemic capsids are engineered, as well as highlight some considerations for their use. Then, we’ll provide...differences occur not just between mice and NHPs; some capsids show both different efficacy and tropism...2023 AAV-PHP.S* PNS, including ENS (broad): somatic and visceral sensory ganglia, type II spiral ganglion...E. E., Mahoney, J. T., Kojima, Y., Ding, Y., Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli...

...lessons to teach, and how — some choose to run full semester-long classes, some pick and choose a few lessons... they remember the moments when they discover something new for themselves and when they effectively contribute...

...infected bacterium can either exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into...factor (F-factor). The F-factor is found on an episome, a piece of DNA that can replicate on its own or... or be integrated within a bacterial chromosome and allows the donor bacterium to make a small “bridge...

... in some systems, rare codons and four-amino acid frameshift codons are also used. Note that some systems... which non-canonical amino acids (ncAAs, also sometimes called unnatural amino acids) are placed in a ...

...hard-to-reach organs, such as the brain. Secondly, some viruses have a low immunogenicity which makes them...Mutagenesis Possible Adenovirus Remains Episomal (Little Risk of Insertional Mutagenesis) High ...Expression High Immunogenecity AAV Remains Episomal (Little Risk of Mutagenesis) Very Low Immunogenicity...

...closer look at the construction and validation of some of these tools created by the Trimmer Lab that will...of the correct clones, it was determined that for some constructs more than 90% of the colony PCR validated...aberrant IgG light chain transcript was expressed by some of the hybridomas and, as a result, subsequently...

...inventory of ready-to-use viral vectors. Here are some of the AAV and lentiviral preps we have released...proteins at the beginning of April. Now, we've turned some of the most requested plasmids into lentiviral preps...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...

...things to come, we wanted to take a look back at some of the contributions from our depositors. Below ...puromycin cassette that makes it less efficient in some cell lines. pSpCas9(BB)-2A-Puro (PX459) V2.0 was...puromycin cassette that makes it less efficient in some cell lines so it may be best to go with pSpCas9(...

... and use of Twitter and LinkedIn probably had something to do with it. Five resources to help you get...post is designed with beginners in mind, there’s some great reminders for Twitter pros as well, like tip...each of the three social media platforms, there’s some statistics on what percent of scientists and engineers...

...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate that...more reproducible and your results more valuable. Some journals, including F1000Research, also ask for ...published. In summary, it is a good idea to take some time and look into which journal would be best suited...

...number by introducing genes of interest into the chromosome. But this has its own set of problems. Rapidly...is expressed from a plasmid as opposed to the chromosome. Let’s take a look at how the stabilized promoters...levels of expression between a plasmid and the chromosome The team also showed that the levels of gene ...

...reporters stain cultured cells and tissue sections, some reporters are better suited for particular applications... 10 HA or 10 MYC tags built into a GFP scaffold. Some GFP nanobodies  stain poorly when tagged with a ...secondary Enzymatic reactions on tissues can be cumbersome Tyramide reagents can be expensive Can only stain...

...to troubleshoot the cloning experiment. Here are some tips that will help you with your cloning project... Before diving into the experimental work, spend some time outlining the construction of the plasmid and...plasmid troubleshooting guide, you hopefully have some new strategies to tackle that tricky cloning project...

..., we wanted to walk through some of our favorite milestones and share some thoughts from the community...began distributing ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, ...

...affinity reagents, and for good reason. However, sometimes you want a tool that is a little more compact ...applications and have similar advantages, though with some caveats.  First off, F(ab’)2 fragments are bivalent...less stable and have lower binding affinity than some of the larger fragments.  Think of antibody fragments...

...infect (or not infect) different cell types with some specificity. There are a lot of AAV serotypes, many...expressed, viral cargo is usually quite stable and in some mammalian tissues has shown expression for several...host genome and therefore the transduced gene is episomal and does not divide and replicate in the same ...

...carry CRISPR circuitry, it can slow their growth. Something has to be done about that.  The second problem...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...genetic designs of that scale could be created. Someone could take on an engineering project including ...

...to quickly identify underlying genetic causes of some human conditions, opening up new avenues for therapeutics...that are responsible for integration into host chromosomes, further minimizing potential activation of innate...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to be as...

...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: The ...hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now ...