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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 716 results for: cas

Showing: 441 - 460 of 716 results

  1. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...World Health Organization says the number of new cases could rise to 10,000 a week within two months. ...has been fatal in 70 percent of the most recent cases, primarily in West African countries. Even that ...

  2. Antibody Guide

    Type
    Guide
    ...expression. Monoclonal antibodies are necessary in cases where extreme specificity is important. Their epitope...when epitope specificity is not a concern or in cases where you may want to identify or collect the protein...azide, as it acts as an inhibitor to HRP. In those cases, thimerosal can be used as an antimicrobial agent... using the above process and try again. In some cases, it may be preferable to buy small aliquots of many...

  5. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...injection site and choosing a specific serotype, but occasionally you may want to further control where your gene...mouse line that contains a Cre-dependent mCherry cassette under the control of an otherwise ubiquitous promoter...

  6. Editor's Choice, August 2016

    Type
    Blog Post
    ...uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at...Viral Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect...

  9. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...genes at the site of insertion. In both of these cases, transposons combine the advantages of viruses and...2) a transposon containing the gene-expression cassette that can translocate within the genome. The Sleeping...

  10. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ... the other posts in this series. Listen to our podcast interview with Connie Cepko to learn about her ...leaders rarely give orders (although they must occasionally). Good leaders exert influence in other ways...

  11. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ... a PI who is a good mentor is important in both cases. As a PhD student you need to learn how to be a ...you pursue any career alternative? Listen to our podcast interview with Harvard Medical School researcher...

  12. RUBY-Red Siliques

    Type
    Blog Post
    ...transgene free individual. For example, with CRISPR-Cas9 gene editing, transformation may be identified by...CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation CRISPR 101: ...

  15. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...non-specific staining can be a challenge in the best of cases. But it can be particularly challenging when your...primary antibody that has your reporter (in this case a fluorophore) directly attached to it. (B) Another...

  16. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out...protein functionality (Arribere, et al). In rare cases, tags can also affect protein localization and solubility...

  17. Plasmid Cloning by PCR

    Type
    Blog Post
    ...of a hairpin structure within your primer. In our case, we will add TAAGCA, resulting in a final Forward...to the sequence to be amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying...

  18. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... common event. What are exosome AAVs? In 2012, Casey Maguire and colleagues from Massachusetts General...28082074. PubMed Central PMCID: PMC5368844.  Maguire, Casey A., et al. "Microvesicle-associated AAV vector as...

  19. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ... strategic knowledge to solve a clinical disease case. As a teacher, you explain the game rules and then...face each other on a single chosen clinical disease case. Then, each team received a whole set of cards (...

  20. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells,... delivering receptor constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are...
Showing: 441 - 460 of 716 results