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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 539 results for: genome

Showing: 441 - 460 of 539 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...generated offspring tomato plants that inherited the genomic edit but not the editing machinery. Together, these...

  4. MXS Chaining

    Type
    Blog Post
    ...dinucleotides are underrepresented in most vertebrate genomes (Josse et al., 1961, Swartz et al., 1962), SalI...

  7. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...cells. In experiments employing gene targeting or genome editing technologies, such as RNAi or CRISPR, non-targeting...

  10. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...directly into this vector and targeted to the AAVS1 genomic safe harbor locus using untagged SpCas9 (Addgene...Zhang lab's PX330 , simultaneously targeting the genomic site and the donor vector. The PITCh donor plasmid...vectors to insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid...-programming sgRNA and HR donors for selectable genome modification. This system can be used to create...

  12. Zinc Finger Consortium Reagents

    Type
    Collection
    ... these reagents are provided below: Zinc Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish...expressing zinc finger FokI nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents...

  13. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...

  14. Retrovirus Plasmids

    Type
    Collection
    ...gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic proteins, ...This provirus then gets integrated into the host's genome by the accompanying integrase protein. When scientists...γ-retroviruses integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional...

  15. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...dCas9-FokI to dimerize at a given region of the genome. Like nickase technology, this technique reduces...

  16. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Plasmids: Yeast Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ... Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering... Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription activator-like...target DNA sequences in living cells (TALE-mediated Genome Visualization [TGV]). pTALYM3 and pTALYM4 contain...this page are designed to target and edit the epigenome of putative enhancers in mammalian cells. These...target locus ( Mendenhall et al., 2013 ). This epigenome editing was also shown to alter regulation of ...

  18. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... prime editor plasmids for (search and replace) genome editing....Prime Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...designed with a spacer that binds to a specific genomic DNA locus and directs the nickase to the target...

  19. AAV Molecular Tools

    Type
    Collection
    ...Affinity Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression Tracers Tetracycline...studying cell ablation. 1, 5 Nirao Shah , Jim Wells Genome Engineering Tools These AAV encode Cas9 nuclease...nuclease used in the CRISPR-Cas9 system for targeted genome editing. ID Name Expression System Activity Serotype...

  20. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...recombinant AAV does not integrate into the host genome, transgene expression can be long-lived. The utility...conjunction with the plasmid that encodes the viral genome (typically called cis -plasmid or transfer plasmid... a general list of plasmids that encode the AAV genome (i.e., the genetic information that will be contained...
Showing: 441 - 460 of 539 results