We narrowed to 534 results for: invs

1. Plasmids 101: Degron Tags

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   ...probably best to avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag...

2. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ...and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence...

3. Cloning Mammalian Cells with the Agarose Method

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   ...two 48-well plates of clones) which enables investigators to identify specific clones with desirable properties...

4. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ...essentially are messed up chromosomes with multiple inversions. They are unable to participate in genetic recombination...

5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   ... yeast (109-1010 members). This selection step involves using a commercial kit for in-vitro translation...

6. Transferable Skills Guide: Managing a Team

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   ... so plan accordingly -  but think of it as an investment: once the person is trained you will have more...

7. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function...

8. Viral Vectors 101: Voltage Indicators

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   ...specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic...

9. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...design and analyze batches of CRISPR samples. We invite you to jump in and try it out, or take a look at...

10. Truncated gRNAs for Regulating Gene Expression

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    ...and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However...

11. Plasmids 101: Knockout/Knock-In Plasmids

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    ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...

12. Plasmids 101: Optimizing Plasmid Yields

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    ...with repeat elements, such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform...

13. Anatomy of a Plasmid Page at Addgene

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    ...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...

14. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...characterized in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency...

15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...that will provide a similar resource for those involved in monocot engineering projects. The MoClo tools...

16. Tips for Using BLAST to Verify Plasmids

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    ...blastn or blastp, due to the additional searches involved in translating the nucleotide sequence into all...

17. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...

18. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...

19. AAVs in Retinal Gene Therapy

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    ...easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged...

20. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular...