We narrowed to 534 results for: invs
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TypeBlog Post... so plan accordingly - but think of it as an investment: once the person is trained you will have more...
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CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...design and analyze batches of CRISPR samples. We invite you to jump in and try it out, or take a look at... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...two 48-well plates of clones) which enables investigators to identify specific clones with desirable properties... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...essentially are messed up chromosomes with multiple inversions. They are unable to participate in genetic recombination... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post... methods add an additional viral passage. This involves taking your first round of virus, called P1 (or... -
Plasmids 101: Degron Tags
TypeBlog Post...probably best to avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...with repeat elements, such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...characterized in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...that will provide a similar resource for those involved in monocot engineering projects. The MoClo tools... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...blastn or blastp, due to the additional searches involved in translating the nucleotide sequence into all... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ... -
A Guide to Starting Your Own Journal Club
TypeBlog Post... wanted to learn more, even those not directly involved in the project. Once we gauged interest from ... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post... that even though these early steps don’t even involve antibodies, they are still critical to the outcome...