Addgene

We narrowed to 532 results for: invs

Showing: 441 - 460 of 532 results

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Viral Vectors 101: Voltage Indicators

Type

Blog Post

...specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic...
AAV Vector Quality Control: Going the Extra Mile with NGS

Type

Blog Post

...and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...two 48-well plates of clones) which enables investigators to identify specific clones with desirable properties...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Type

Blog Post

...essentially are messed up chromosomes with multiple inversions. They are unable to participate in genetic recombination...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

...and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...
Transferable Skills Guide: Managing a Team

Type

Blog Post

... so plan accordingly - but think of it as an investment: once the person is trained you will have more...
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Type

Blog Post

...design and analyze batches of CRISPR samples. We invite you to jump in and try it out, or take a look at...
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Type

Blog Post

... yeast (109-1010 members). This selection step involves using a commercial kit for in-vitro translation...
Fluorescent Tagging of Endogenous Genes with SapTrap

Type

Blog Post

...spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function...
Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

...with repeat elements, such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform...
Anatomy of a Plasmid Page at Addgene

Type

Blog Post

...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...
CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

...characterized in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

...that will provide a similar resource for those involved in monocot engineering projects. The MoClo tools...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...blastn or blastp, due to the additional searches involved in translating the nucleotide sequence into all...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

... that even though these early steps don’t even involve antibodies, they are still critical to the outcome...
AAVs in Retinal Gene Therapy

Type

Blog Post

...easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged...
Transferable Skills Guide: Identifying Your Transferable Skills

Type

Blog Post

... for working with lots of students, managing inventories in the lab, or organizing a research project,...
SciComm: Taking Science to Elementary and Middle Schools

Type

Blog Post

...interactive. If you can think of a good analogy that involves student participation, make it happen! How should...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular...

Showing: 441 - 460 of 532 results

Results per page:

Viral Vectors 101: Voltage Indicators

AAV Vector Quality Control: Going the Extra Mile with NGS

Cloning Mammalian Cells with the Agarose Method

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Truncated gRNAs for Regulating Gene Expression

Plasmids 101: Knockout/Knock-In Plasmids

Transferable Skills Guide: Managing a Team

CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Fluorescent Tagging of Endogenous Genes with SapTrap

Plasmids 101: Optimizing Plasmid Yields

Anatomy of a Plasmid Page at Addgene

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Tips for Using BLAST to Verify Plasmids

Deep Dive: Fixing and Permeabilizing for Immunofluorescence

AAVs in Retinal Gene Therapy

Transferable Skills Guide: Identifying Your Transferable Skills

SciComm: Taking Science to Elementary and Middle Schools

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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