We narrowed to 883 results for: prot

...capsid proteins that determine the AAV serotype. During transduction, the viral capsid proteins bind to... to the target cell’s surface proteins and this interaction determines the specificity and efficiency ...

...researchers from some labs have developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine...the new dLight1 sensor is a single fluorescent protein-based sensor. This sensor was engineered from a...Learn about Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research...

...Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers...fluorescent tags and activation of light sensing proteins. Furthermore, during the first 2 weeks of life...functions through opsins which are light sensitive proteins. Opsins used in optogenetic studies are not native...

... in any way. For example, if green fluorescent protein (GFP) was the reporter gene and green cells were...be something phenotypic (such as a fluorescent protein or something species-specific) or a selectable ...what remains to be discovered in the eukaryotic proteome? Open Biol. (2019) 9:180241. DOI: https://doi....

...Pages Basic Molecular Biology Protocols Diagnostic Restriction Digest Protocol ... tags, origins of replication, and fluorescent proteins that are already included in Snapgene’s extensive...

...bacteria. Until recently the only available Cas9 proteins were isolated from mesophilic (medium heat loving... as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at high temperatures, so to use...) database, Harrington et al discovered a Cas9 protein from the thermophilic bacteria Geobacillus stearothermophilus...

...the adenoviral helper plasmid which contains the proteins needed for the virus to replicate,  and the transfer...include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of ... Find AAVs for your experiment Browse our AAV protocols ...

...including only titration of AAV particles and a protein stain to assess purity. For labs that produce a... blog post on AAV titers Browse viral vectors protocols and tips Resources on Addgene.org Find AAVs ...Learn more about Addgene's viral service Find protocols for working with virus ...

...10 kb) or has a low copy number, try following protocols for low copy plasmids. For example, using a longer... intact plasmid. Please see our gel extraction protocol for more details.   Figure 2: An uncut... Find more molecular biology and viral vector protocols Learn about the common lab E. coli strains Find...

...version at Addgene! Fluorescent protein and control AAV These fluorescent protein expression vectors can be ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...goal is to provide scientists with new tools and protocols that they can easily incorporate into their own...viral particle’s affinity for heparan sulfate proteoglycans on the surface of the packaging cells (Vandenberghe...service Learn about viral production Find viral protocols here ...

...These kits provide in-depth, ready-to-teach lab protocols and supporting materials such as instructional...conditions, to guiding students through specific protocols, ABRC staff are available via e-mail and phone...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants Parts Kit...

...serotypes that can be used to deliver fluorescent proteins to cells as determined by the serotype. You can... viral vector pages as well as expanded virus protocols, information on how we produce and test virus,...Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

...this post. 3. Plasmids 101: E. coli Strains for Protein Production Addgenie Julian Taylor-Parker explains...might want to use a specialized E. coli strain for protein production and gives a quick rundown of some of...of the strains you can use to express your protein of interest. 4. Plasmids 101: Blue-White Screening ...

...immunoassays due to only having one binding site per protein. Having multiple antibodies allows you to use them...light over the last decade, but the fluorescent proteins (FPs) necessary for many of these applications...Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy. Nat Methods 19, 1612–...

...Besides being compatible with any vector, the SLIC protocol itself is flexible. For one thing, those 5′ overhangs...boosts efficiency with the homologous recombination protein RecA. (It’s effective with as little as 3 ng of...Resources at Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing a Molecular...

...combine computational processing with biological protocols and revolutionary physical approaches. However...the structure in a similar way to the biological protocol, and assembly designs based around the use of ...commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC...

...changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying .... 2). These systems allow for rapid continuous protein evolution through many rounds of mutations, selection...rate Phage infection results in a complete ABE protein. If the phage delivers an active variant of the...

...that EvolvR could also be used to map protein-protein interactions, investigate non-coding segments of the...understanding of the key residues responsible for this protein-drug interaction.   The Dueber Lab propose that...

...explore how engineering fields rely on rapid prototyping to develop and test hypotheses. Students then...then use cellular extracts as a means to rapidly prototype DNA circuits and logic gates. This BioBuilder-...projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub teams’ biological ...