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1. In Vivo Biotinylation of Bacterial Fusion Proteins

   Type

   Blog Post

   ...well-controlled. The lack of site selectivity typically diminishes the apparent affinity for the ligand, especially...

2. Addgene Begins Distribution of Recombinant Antibodies

   Type

   Blog Post

   ...Bandrowski et al., 2016). It’s also been well established that recombinant antibodies are excellent for...

3. What Do I Do Now? Academic v. Non-Academic Career Decisions

   Type

   Blog Post

   ..., interests and passions is another great way to shed light on what paths to pursue. Spend some time really...

4. Fluorescent Biosensors for Measuring Autophagic Flux

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   ...oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org...

5. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

   Type

   Blog Post

   ...nts, and is thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS...

6. Plasmids 101: Origin of Replication

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   Blog Post

   ...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic...

7. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   ...Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu & Chunyu Han. “DNA-guided...

8. Plasmid Cloning by PCR

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   Blog Post

   ...check out that post if you need a more detailed refresher on the downstream steps. For this example, we...

9. Another Pathway into Cells: iTOP

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   Blog Post

   ...protein purification (once a protocol has been established) may be less laborious than viral purification...

10. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post

    ...promise to be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and...

11. The MTA at Addgene

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    Blog Post

    ...One-Time Plasmid Deposit and Distribution Agreement establishes the terms under which your material will be distributed...

12. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    Blog Post

    ...amplification, refer to the Zhang lab’s previously published protocol and its relevant modifications on the...

13. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    Blog Post

    ...properties. Find these voltage biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ...

14. The Importance of High Titer for AAV Transductions

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    ...definitely also matters). Check out this post for a refresher on AAV titers Making contact with a cell Although...

15. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post

    ...experiment, the DNA sample being sequenced must be sheared into smaller fragments that are then barcoded for...

16. Creating accessible biology activities in schools with BioBits

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    Blog Post

    ...freeze-dried into pellets (FD-CF pellets), leaving them shelf-stable until water is added and protein production...

17. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post

    ...Bulina, M. E., Chudakov, D. M., Britanova, O. V., Yanushevich, Y. G., Staroverov, D. B., Chepurnykh, T. V.,...

18. Visualizing Translation at the Single Molecule Level

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    Blog Post

    ...causing it to appear yellow. The act of translation pushes NLS-PCP-GFP off of the mRNA, leaving only a red...

19. Summer SciComm Series: A PhD in Science Communication

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    Blog Post

    ... BSc in Anatomy, the University of Otago had established the Centre for Science communication and I was...

20. Plasmids 101: Gram Negative and Positive Bacteria

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    Blog Post

    ...stain within their membrane, while the stain is washed from the gram negative bacteria’s thinner membrane...