We narrowed to 545 results for: SEC

1. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   ... a convenient most requested filter. Applying a second filter, this one from the ‘Requests’ category, ...

2. Plasmids 101: Colony PCR

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   Blog Post

   ...plasmid backbone. Backbone-specific primers: A second option is to design backbone-specific primers. ...

3. CRISPR Antimicrobials

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   Blog Post

   ...targeting 1) the given resistance gene and 2) a second lytic phage. Bacteria that receive this phage are...

4. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   ...single-pass two-hybrid screening (6). See the Tips section for more on how to increase your rate of interactions...

5. Hot Plasmids Spring 2024

   Type

   Blog Post

   ...duplicated during lentiviral integration, producing a second copy of the sgRNAs. Image reused from Walton et...

6. Antibodies 101: Reading a Flow Plot

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   Blog Post

   ...because we usually assume (0,0) to be at the intersection of X,Y. For flow plots, however, that is generally...

7. Transferable Skills Guide: Career Planning Resources

   Type

   Blog Post

   ...job, you’ll be in the best possible position to secure it! Further Reading and Outside Resources http...

8. Career Insights: Technical Support Specialist

   Type

   Blog Post

   ...less technically challenging. Alongside there is a second-line team that takes on cases requiring in-depth...

9. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   ...DNA sequence and the interior target sites. In a second step, the two compatible target sites are cleaved...

10. Hot Plasmids - August 2020

    Type

    Blog Post

    ...exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...

11. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    ...PHP.B transduction as their C57BL/6J parent. The second generation (F2) of outbred mice also showed a Mendelian...

12. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

    ... Thus, CRISPRa represents a powerful method to dissect the individual and shared contributions of transcript...

13. CRISPR 101: Any Base Transversion Editors

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    Blog Post

    .... ABE Adenine Base Editor Converts A → G; the second type of base editor invented. CGBE C → G Base...

14. Neuronal labeling with Spaghetti Monster

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    Blog Post

    ...channel. In multi-color imaging experiments, the second color is usually provided by red fluorescent proteins...

15. Supporting Reproducibility with a Connected ELN

    Type

    Blog Post

    ...s possible to link between various documents. A second example is the integration between RSpace and Slack...

16. CRISPR-mediated Plant Base Editors

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    Blog Post

    ...uracil glycosylase inhibitor (UGI) minimizes the second outcome and increases the generation of the desired...

17. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    ... cells that have removed the cassette through a second recombination event between the two flanking repeats...

18. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    ...The first generation of CRISPR could cut DNA, the second generation could do base editing, and with the ...

19. Viral Production at Addgene

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    Blog Post

    ...page for the corresponding catalog item (see maps section for images).     Figure 2: AAV Pro cells...

20. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    ...associated with the library and reading their methods sections to see what worked.  Ready, optimize, transduce...