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We narrowed to 1,076 results for: TOR;

Showing: 691 - 720 of 1076 results

  1. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...- e.g. codon choice may interrupt transcription factor binding sites) As you might imagine, it’s not ...not easy for humans to balance all of these factors on their own. Luckily, many researchers have created...Kimberly A., et al. "Selective charging of tRNA isoacceptors induced by amino‐acid starvation." EMBO reports...

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...research scientists, faculty, students, private sector researchers, or pretty much anyone applying the...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...is not hard either. Here are some resources: ImpactStory, a website that helps researchers go beyond citation...

  3. Five Popular Model Organisms

    Type
    Blog Post
    ... al., 2009). By injecting mice with retroviral vectors that harbored different mutations found in leukemia...Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally control gene expression...discovery of RNAi in the worm actually rewarded depositors Andrew Fire and Craig Mello a Nobel Prize in ...

  4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed a version of Tetbow...Livet, Jean, et al. "Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous...Markus Grompe. "Clonal tracing of Sox9+ liver progenitors in mouse oval cell injury." Hepatology 60.1 (...

  5. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...CRISPR, RNAi methods are often quicker and the repositories of validated small RNAs are vast and difficult...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186...

  6. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...too many activities too close to one another. Monitoring task progress. For this, I always make sure I... lists. Because I know what my goals are (see monitoring task progress), and I know that I’ve blocked ... give it a final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly...

  7. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... the microflora that reside there. Biotin is a cofactor that in its active form is covalently attached...consists of a biotin molecule attached to a photoreactive arylazide group. Under strong visible light,...single-step purification of tagged transcription factors in mammalian cells and transgenic mice.” PNAS 100.13...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter... addition to the “A” site already in the parent vector). Also shown are the “B” restriction sites, which...Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment ...

  9. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 ...Guttman A, Thompson L. Vaccine Plasmid Topology Monitoring by Capillary Gel Electrophoresis. Curr Mol Med... JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream...

  10. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory experience with peptide...knockout line for validation, please view this tutorial from Stuart Orkin's and Daniel Bauer's labs.  ...

  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...modular toolkits build single plasmid targeting vectors that encode the gRNA transcript and the repair ...recognized by the 2020 Nobel Prize in Chemistry), and Victor Ambros and Gary Ruvkun for their seminal work on....114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc-119+ selection...

  13. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...Now we have over 8,000 CRISPR plasmids in the repository that have been shared over 140,000 times to over... today, we’ll start with a CRISPR overview and history. Tuesday will focus on generating knockouts using...

  14. The Twelve Days of CRISPR

    Type
    Blog Post
    ...first generation of base editors to the eighth generation (and dual base editors, too!).  On the seventh...

  15. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the...

  16. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...lab process. Along with our role as a plasmid repository, Addgene aims to be a vital educational resource...the Addgene Blog 5 Science Rap Videos and Their Creator: Tom McFadden SciComm with the Experts at Science...

  17. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...received an award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that will help...23:44 : Follow up Kingdom's PhD projects - HIV inhibitors, biofuels, challenges in Malawi, and more 23:...

  18. Call for Guest Bloggers

    Type
    Blog Post
    ...purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists A new plasmid...do you get out of it? Writing Experience - Our editors will work with you to help hone your blog post ...

  20. CRISPR Cheat Sheet

    Type
    Blog Post
    ... a functional change in the DNA sequence. Base Editors: Modified versions of CRISPR nucleases that make...sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases that cut or modify RNA as opposed...

  21. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...Pathology at Masachusetts General Hospital and Postdoctoral Fellow in the Church and Collins labs at the... Blog Learn More About Alex's Work with Cas9 Activators Michael Koeris Explains His Path from Grad Student...

  22. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...introduce the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General Counsel at Atalanta...really just taking off. I found out that patent attorneys with a biology background were in demand. Q: Can...

  23. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ...main topics of the blog: Plasmids CRISPR Viral Vectors Fluorescent Proteins Science Careers You can use... us in the comments below. We’ll track down the story, get the inside scoop, and keep you up-to-date. ...

  25. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...ineffective or unimportant. With so many big contributors to the sustainability problem, how can one little... even has a list of biological samples that are stored long term at -70 °C. My Green Lab also has resources...

  28. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...their gene of interest into a previously deposited vector - then you'll find the plasmids listed below the...like to contribute plasmids you have made to the repository, email us and we'd be happy to help. Read More...

  29. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...Cell demonstrate how PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise and endurance...altogether. Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors...

  30. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an ...home message was that being creative and telling a story makes scientific topics both interesting and approachable...
Showing: 691 - 720 of 1076 results