We narrowed to 1,029 results for: ELL

1. Plasmids 101: 5 factors to help you choose the right cloning method

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   ...from the PCR process (the precise mechanism isn’t well understood). Finally, TOPO cloning is another good...come with comprehensive and tested protocols, as well as premixed and ready-to-use reagents. Thermo Fisher...

2. Twenty Years of Addgene Sharing: CRISPR

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   ...year from 2012 to 2023.  What does this tell us? Well, it’s hard to draw any exact conclusions, but...

3. Polymerase Chain Reaction Overview and Applications

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   ...late 1960s during research at hot springs in Yellowstone National Park.  In addition to Taq DNA polymerase... Alyssa Cecchetelli...

4. Common Injection Routes in Mice

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   ...different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers... injections are most often used when injecting a cell suspension to create xenograft models. Restraint...

5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   ... smaller cargos, and the ability to easily cure cells of the plasmid after integration. Figure ...into the mini-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically...

6. Transferable Skills Guide: Identifying Your Transferable Skills

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   ...collaborators, or co-workers want to know that you are a well-rounded individual with more going for you than ...! Enjoy that feedback, bask in the glow of a job well done, take some time to think, and write your thoughts...

7. The Importance of a Fun Workplace: Company Culture at Addgene

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   ...conversation to get people excited!    Laura Veckerelli is the Administrative and Operations Coordinator... Laura Veckerelli...

8. Supporting Reproducibility with a Connected ELN

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   ...access, review, and query. Gates Open Research and Wellcome Open Research both accept deposit of data associated...with two of the five: Figshare and Dataverse, as well as the DSpace repository. These integrations utilize...

9. Healthcare Consulting: A Door to the Business of Life Sciences

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   ...’ll also talk about the skills you’ll need to do well as a healthcare consultant and what you could do...the challenge at hand and help resolve it through well-informed recommendations. Healthcare consulting ...

10. Site Directed Mutagenesis by PCR

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    ...contributed by guest blogger, Kristian Laursen from Cornell University. Site directed mutagenesis is a highly... Laursen! Kristian Laursen is a researcher at Cornell University.       References 1. Breslauer, Kenneth...

11. How to Deposit Your Plasmids with Addgene

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    ...notation. Please indicate the amino acid change as well as any functional consequences of that change. For...for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression). This...

12. 2022 Depositor Week

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    ...Hello and welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social...

13. Suggest A Plasmid You'd Like to Find at Addgene

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    ... but were unable to find the plasmid at Addgene? Well, we’re here to help with our Suggest a Plasmid Campaign...

14. Addgene's 20th Anniversary Party

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    ...group of talented individuals that makes Addgene — well, Addgene. We're so grateful for each and every one...

15. The 12 Days of CRISPR: 2021

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    ... to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth day of CRISPR, ...

16. Trends in CRISPR and SynBio Technologies

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    ... of open-source distributors such as Addgene, as well as a number of online user forums such as http:/...

17. New FLIS Plasmid (April Fools 2022)

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    ...tend to come with a rather astonishing ability to...well, annoy. Fly-related Issues in the Lab A significant...

18. New Videos: Addgene Lab Tips

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    ... etiquette that will keep the lab running like a well-oiled machine. Lab tip #1: Glove trick Putting on...

19. Addgene at SfN23!

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    ...The Booth What will you find at Addgene's booth? Well, first, Addgenies! We'll have an antibody specialist...

20. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

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    ...interview with synthetic biology researcher Tom Ellis Resources at Addgene.org Find CRISPR Plasmids ...

21. Expanded Access to Rabies Plasmids

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    ...average. Therefore, we recommend placing your order well in advance whenever possible, as we cannot expedite...

22. DIY DNA Ladders from Penn State University

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    ...commercially available ladders, the 100 bp ladder works well for both agarose and native polyacrylamide gels....

23. Twelve Amazing Guest Blog Contributors!

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    ...Addgene. An Addgene Summer Internship Matteo Tardelli, who added to our career advice collection. Embracing...

24. Bringing Renewable Energy to Addgene

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    ...styrofoam and plastic recycling programs in the lab as well. For a more in-depth look at Addgene’s current lab-oriented...

25. Podcast: A Malawian Professor's Path to Biotech Research

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    ...University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata now works as a lecturer in molecular...

26. Call for Guest Bloggers

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    ...Your Favorite Fluorescent Protein Oligomerizes in Cells   ...and many more! Sign up to write for the Addgene...

27. Our New Antibody Mascot is....

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    ...introduce their new friend…  Fig. 1: Meet...well, frankly, we're not sure yet!   This as-yet–...

28. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

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    ...decorating skills. To join the contest, simply embellish and adorn your lab space or your work from home...

29. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

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    ... time in PubMed searching for research articles? Well then, my scientist friend, do I have a handy tool...

30. Celebrating One Million Plasmids Shared!

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    ...scientists sharing their contributions on social media as well the excitement amongst our Addgenies on social media...

31. New Viral Vectors - Summer 2024

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    ...useful for both in vitro and in vivo work with many cell types, as CAG is a ubiquitous and constitutive promoter...

32. Interview with Bart Newland, Addgene’s Newest Board Member

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    ... a biology background were in demand. Q: Can you tell me a bit about your job currently? What is your ...

33. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

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    ... that work in under-funded fields. Like Louis Bengyella and Kwabena Duedu who were awarded similar grants...

34. Editor's Choice, October 2016

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    ...,” Future of Research executive director, Gary McDowell, gives a thorough yet concise rundown of how recently...

35. Grad School Advice Part 1: Picking a Lab and a Project

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    ...Genomics at Harvard Medical School, and is currently a fellow in clinical genetics at the University of California...

36. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

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    ...biology research in the labs they’re granted to, as well as shine a light on some of the great minds working...

37. Grad School Advice Part 2: Building Community

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    ...Genomics at Harvard Medical School, and is currently a fellow in clinical genetics at the University of California...

38. Fueled by Coffee at #SfN14

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    ..., many lattes and espressos. The energy boost is well needed to keep up with all the awesome talks, symposiums...

39. 2022 and 2023: Reflections and Looking Forward

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    ...upon, a wealth of history to learn from, and a compelling future to plan in the changing landscape of science...

40. Making Career Development an Integral Part of the Science Training System

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    ...training programs. In 2018, the ASBMB and Burroughs Wellcome Fund provided seed funding to take this initiative...