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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 986 results for: IGH@;

Showing: 921 - 960 of 986 results

  2. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...purified AAV. Option #3 Puncture the QuickSeal tube slightly below the 60–40% interface with an 18 ga needle... (1999): 973-985 (Link opens in a new window) . Right panel: cartoon indicating the position of the needle...harvesting of the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers... viral vectors," (Link opens in a new window) copyright (2006). Figure 3: Example of an SDS-PAGE gel of...

  10. Plasmid Cloning by PCR

    Type
    Blog Post
    ...amplify your insert DNA. It is important to use a high fidelity polymerase to minimize mutations. The fidelity...

  12. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds...

  20. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...transfection into mammalian cells is fairly straightforward and the resultant cells can either express ...

  21. Lentivirus ddPCR Titration

    Type
    Protocol
    ...viral particles. Users may need to run lower or higher dilutions depending on their particular sample....tissue culture treated dish, VWR, 29442-042 DMEM, high glucose, pyruvate, Corning, 10-013-CV Heat-inactivated... Reagent Preparation DMEM Complete: 500 mL DMEM, high glucose, pyruvate 55 mL heat inactivated premium...increases by a factor of ~2 as you progress from the higher dilutions to the lower dilutions (blue). As shown...

  24. Antibodies 101: Isotypes

    Type
    Blog Post
    ... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ...

  27. DNA Quantification

    Type
    Protocol
    ...spectrophotometer uses the absorbance/transmission of light through a liquid to determine the concentration ...liquid. Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength... absorbance, you need to know the wavelength of light that your substance maximally absorbs. In the case...

  28. Summer SciComm: Preprints

    Type
    Blog Post
    ...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their...

  31. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...

  32. Working with Nuclear Receptors

    Type
    Blog Post
    ...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...
Showing: 921 - 960 of 986 results