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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,006 results for: RING

Showing: 921 - 960 of 1006 results

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988...

  13. Plasmids 101: Protein tags

    Type
    Blog Post
    ...FLAG) are cleaved by specific proteases without requiring the addition of an independent cleavage recognition...

  18. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ..., and it is exchanged with the gene of interest during the BP or LR reactions. Since the CcdB protein ...

  24. Bioinformatics at Addgene

    Type
    Blog Post
    ... reviewing and testing several platforms for engineering pipelines, we selected Nextflow, a workflow management...

  25. Cloning

    Type
    Guide
    ...Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson ...are both blunt-ended, can then be fused together during a ligation reaction with T4 DNA ligase. Restriction...easy to add restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested...Because of the relatively long stretches of base pairing in the annealed product, ligation is rendered unnecessary...

  26. Five Popular Model Organisms

    Type
    Blog Post
    ...of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies are also...

  30. Adenovirus Guide

    Type
    Guide
    ...elements into the genome, often altering gene activity. Oncolytic Referring to the destruction of cancer ...HER911 cells throughout viral vector production. During experiments, GFP can be used to sort cells infected... Adenoviral vectors are excellent vectors for delivering target antigens to mammalian hosts and have been...

  35. Plan Your Experiment

    Type
    Guide
    ...sites are called off-targets and should be examined during gRNA design. In general, off-target sites are not...gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...Publications Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...

  36. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...markers, such as the puromycin resistance gene, conferring antibiotic resistance to infected host cells....Retroviral Vector Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin...by high-speed centrifugation for CAR T cell manufacturing. Molecular Therapy — Methods & Clinical Development...

  37. Science Guides

    Type
    Guide
    ...system in bacteria, have been modified for genome engineering. Find essential background information on CRISPR...Optogenetics Optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons...

  38. Addgene's eBook Collection

    Type
    Guide
    ...protein; information on FRET, biosensors, and monitoring protein localization; an introduction to non-...the many career options in science, advice on mentoring and management, resources for identifying your...

  39. Guide to Using Pooled Libraries

    Type
    Guide
    ...the library (electroporation and maxiprep). If delivering as virus, make virus; this creates a pooled lentiviral...Viral Vector resources . Check out our blog posts featuring some of our most popular libraries! Genome-wide...

  40. Promoters

    Type
    Guide
    ...Addgene's molecular biology reference material covering promoters, transcription and the transcription...the adaptor molecules that are responsible for bringing amino acids to the ribosome when proteins are ...
Showing: 921 - 960 of 1006 results