We narrowed to 619 results for: PROC

1. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...lines and use these lines to study how immune processes and biological pathways are altered in PMM2-CDG...

2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   ...vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV...

3. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   ...maintain your parental strains throughout this process. In the F1 and later generations, mice that are...

4. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...activation across multiple loci was an arduous process. When using zinc finger proteins or TALE proteins...

5. Corralling and Cornering the Goldilocks Antibody

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   ...Schematic of the goldilocks antibody selection process The exposure time of the primary antibody compared...

6. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   ...pair. In a sandwich ELISA, the first step of this process is the addition of a detection antibody (typically...

7. Viral Vectors 101: Calcium Sensors

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   ...to your parameters.   Kinetics How fast is the process you would like to monitor? Neurons, for example...

8. High Complexity Golden Gate Assembly with NEB’s DAD

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   ...genomes, it required a very stringent selection process and benefited from a longer reaction time of 15...

9. Antibodies 101: Choosing the Right Isotype

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   ...have been cross-adsorbed. The cross-adsorption process filters out off-target antibodies increasing specificity...

10. Hot Plasmids and Viral Preps - January 2021

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    ...active site. The RuvC active site in Casɸ can both process crRNA and carry out the crRNA-guided DNA cutting...

11. Engaging with science and society at pgEd

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    ... and as societal participants in the political process. My role at pgEd I have always been very interested...

12. Adapting Toehold Switches to Detect Zika Virus

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    ...technology through product development, the regulatory process, and manufacturing so that we can get the diagnostics...

13. 5 Tips for Troubleshooting Viral Transductions

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    ...volume. Always remove packaging cell debris before proceeding to ultracentrifugation to avoid contaminating...

14. Cpf1 Update: Comparison to Cas9 and NgAgo

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    ...overhangs. Exploring the efficiency of these two processes will help us better understand where Cpf1 fits...

15. Plasmids 101: FLEx Vectors

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    ...SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites or the...

16. Tips for Titering Your Lentiviral Preps

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    ...subcloning, expansion, and confirmation. This procedure can take several months but should be considered...

17. The Materials Science of Optogenetics Experiments

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    ...animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic ...

18. How-to: 5 Steps to a Great Panel Discussion

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    ... Your audience may have a lot of real or self-proclaimed experts on the topic at hand. You can use this...

19. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    ... thing into a marketable product. During this process, we had an idea that survived the literature review...

20. 10 Basic tips for mammalian cell culture

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    ...bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. ...