We narrowed to 761 results for: Rest

1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ... allows synthetic biologists to use Type IIS restriction sites to easily assemble multiple DNA fragments...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no...

2. New Tool for Lineage Tracing: The ClonTracer Library

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   ...When we developed this tool, there were so many interesting questions that we could apply [it to].” Using...Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned about tumor ...

3. Rabies and Neuronal Tracing

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   ...the G receptor. In neurons, the G receptor is restricted to presynaptic nerve terminals (Lafon 2005). ...applications Use of G deleted RABV in conjunction with restricted TVA and G protein expression is a powerful development...

4. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   ...plasmids are also used to teach students about restriction mapping and western blotting. Students who participate...genetics at Boston College. She is particularly interested in strategies to incorporate research into undergraduate...

5. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   ... CRY2 and CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation...reversible oligomerization when fused to a protein of interest. Fusion protein oligomerization happens within...

6. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ...SpCas9. gRNA A gRNA targeting a DNA sequence of interest is expressed in 293T cells and loaded into Nanoblades...transduction for most cell types they tested. Interested in using Nanoblades? Plasmids are available from...

7. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...particles are delivered into the brain region of interest. AAV or lentivirus?  Both AAV and lentiviral vectors...however, the spreading of vector to the area of interest may be very limited with this method, especially...

8. Plasmids 101: Modular Cloning

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   ...from Golden Gate Cloning, in which a type IIS restriction enzyme and a ligase are used to assemble multiple...described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have to start from ...

9. Hot Plasmids and Viral Preps - January 2021

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   ...scientists can control expression of their genes of interest. While recombinases are powerful gene expression...cells to identify cells exhibiting properties of interest. However, FACS is unable to spatially and temporally...

10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...on a guide RNA complementary to the sequence of interest. Upon binding, CRISPRa systems recruit transcription...having multiple copies of VP64 at each loci of interest, this allows more transcriptional machinery to...

11. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...take me. I’m also just learning. I have a huge interest in data science,” which she’s taking some classes... hours in the lab, she told me, “I was really interested to learn more when I was introduced to bioinformatics...

12. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    .... One popular approach is to fuse a protein of interest to each part of a split fluorescent protein (FP...the behavior of the split FP in your system of interest. Remember that the FP fragments themselves can...

13. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...considerations: Design your gRNA to overlap the CpG of interest. Having the CpG directly inside the gRNA sequence...but if this is not possible due to gRNA design restrictions, anything within 5 base pairs should work well...

14. Viral Vectors 101: Calcium Sensors

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    ...noise and ensure you are measuring the events-of-interest. Sensors suitable for ER measurements include:...scientists to focus on specific cell populations of interest. Many of the calcium sensors described are also...

15. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ..., quantify, deplete, and visualize proteins of interest (Greenfield, 2014). Traditionally, they have been...similarities to host proteins. After the antibody of interest has been cloned into an expression plasmid, the...

16. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    ...outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology park...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

17. Illuminating Epigenetics with A FRET Based Biosensor

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    ...in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...construct specifically to the histone protein of interest (in the figure above, H3 at K9 or K27). Joined...

18. The Materials Science of Optogenetics Experiments

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    ... are all wired up and expressing your opsin of interest, it’s time to get to the fun stuff: the experiments...Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory in opioid...

19. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...student at Harvard University. Do you have a gene of interest but have run into a wall trying to study it? It...the Classical Genetics Simulator website. One interesting thing to know is that males do not recombine ...

20. Viral Vectors 101: Voltage Indicators

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    ...selected to preferentially target your cell type of interest. Viral pseudotyping is also a powerful tool that... optimize them for targeting your cell type of interest. For in vivo imaging applications, the ability...