...things to come, we wanted to take a look back at some of the contributions from our depositors. Below ...puromycin cassette that makes it less efficient in some cell lines. pSpCas9(BB)-2A-Puro (PX459) V2.0 was...puromycin cassette that makes it less efficient in some cell lines so it may be best to go with pSpCas9(...

... and use of Twitter and LinkedIn probably had something to do with it. Five resources to help you get...post is designed with beginners in mind, there’s some great reminders for Twitter pros as well, like tip...each of the three social media platforms, there’s some statistics on what percent of scientists and engineers...

...closer look at the construction and validation of some of these tools created by the Trimmer Lab that will...of the correct clones, it was determined that for some constructs more than 90% of the colony PCR validated...aberrant IgG light chain transcript was expressed by some of the hybridomas and, as a result, subsequently...

...inventory of ready-to-use viral vectors. Here are some of the AAV and lentiviral preps we have released...proteins at the beginning of April. Now, we've turned some of the most requested plasmids into lentiviral preps...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...

...subgroup of researchers in your area of expertise? Sometimes, the funders of your research may stipulate that...more reproducible and your results more valuable. Some journals, including F1000Research, also ask for ...published. In summary, it is a good idea to take some time and look into which journal would be best suited...

..., we wanted to walk through some of our favorite milestones and share some thoughts from the community...began distributing ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, ...

...affinity reagents, and for good reason. However, sometimes you want a tool that is a little more compact ...applications and have similar advantages, though with some caveats.  First off, F(ab’)2 fragments are bivalent...less stable and have lower binding affinity than some of the larger fragments.  Think of antibody fragments...

...to troubleshoot the cloning experiment. Here are some tips that will help you with your cloning project... Before diving into the experimental work, spend some time outlining the construction of the plasmid and...plasmid troubleshooting guide, you hopefully have some new strategies to tackle that tricky cloning project...

...number by introducing genes of interest into the chromosome. But this has its own set of problems. Rapidly...is expressed from a plasmid as opposed to the chromosome. Let’s take a look at how the stabilized promoters...levels of expression between a plasmid and the chromosome The team also showed that the levels of gene ...

...reporters stain cultured cells and tissue sections, some reporters are better suited for particular applications... 10 HA or 10 MYC tags built into a GFP scaffold. Some GFP nanobodies  stain poorly when tagged with a ...secondary Enzymatic reactions on tissues can be cumbersome Tyramide reagents can be expensive Can only stain...

...infect (or not infect) different cell types with some specificity. There are a lot of AAV serotypes, many...expressed, viral cargo is usually quite stable and in some mammalian tissues has shown expression for several...host genome and therefore the transduced gene is episomal and does not divide and replicate in the same ...

...carry CRISPR circuitry, it can slow their growth. Something has to be done about that.  The second problem...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...genetic designs of that scale could be created. Someone could take on an engineering project including ...

...the plasmid to stop functioning properly or be something completely different than what was originally ... other critical functional components. Fun fact! Some older plasmids in our collection do not have a full...based on SnapGene’s “Common Feature” library — with some additional features added by the QC team — and allows...

...to quickly identify underlying genetic causes of some human conditions, opening up new avenues for therapeutics...that are responsible for integration into host chromosomes, further minimizing potential activation of innate...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to be as...

...forward to where it is today. Below we will review some of the CRISPR-based editing therapies that are currently...CRISPR CAR-T treatments for leukemia, lymphoma, and some solid tumors are currently in early-phase clinical...CRISPR-HIV therapy currently in clinical trials is somewhat similar (Hussein et al). It employs Cas9 to cut...

...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: The ...hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now ...

...methods vary in their transcriptional activators: some methods rely on fusion proteins while others re-... exhibits modest levels of gene activation, with some genes experiencing around 2-fold activation levels...method has shown higher activation levels than SAM in some loci across Drosophila cell lines (Sajwan et al....

...patterns throughout the project where it solidified some concepts that are heard often during undergrad. ...huge interest in data science,” which she’s taking some classes on and hoping to pursue. The choice to not... does. Her parents told her that if she found something she loves to do, she would never work a day in...

... initiating the desired cleavage event, although some engineered terminators come close (>95%). For most...presence of the poly(A) tail targets the RNA to the degradosome, which contains enzymes that cut RNA not protected...types of RNA: Polymerase I is responsible for ribosomal RNA, Polymerase II is responsible for mRNA and...

...and sequencing is being organized and funded by someone else (KittyBiome has been incorporated as a non-profit...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to the kittybiome...And if not, I suspect you can find a makerspace somewhere near you. The place to start looking for your ...