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We narrowed to 672 results for: abo.1

Showing: 461 - 480 of 672 results

  1. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169. Soo, Rochelle M., et al... Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous...

  2. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...resume that will give you a chance at an interview:  1) Have an excellent resume ready to be submitted The... your strengths in your resume and don't be shy about asking for help. The worst thing that can happen...

  3. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...Kan, SpHIS5, or CaURA3 selection marker. Lee et al.(1) assessed many of these fluorescent tags in S. cerevisiae...strain. In addition to the collections featured above, many other modular yeast tagging systems have been...Addgene would love to hear from you, our community, about your experience with yeast genome modifications....

  4. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ... everyone was very approachable.     Fig. 1: Examples of DNA sequencing QC work    In addition...intimidating to me, as I had a hard time thinking about how I could fill this time. I was relieved to find...attend to other commitments, without feeling guilty about missing work. As long as my work was finished on...

  5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...depends on what you are looking to study. Figure 1: A schematic showing the differences in chemogenetics...Additional resources on the Addgene blog Read more about optogenetics New to viral vectors? Browse our Viral...Viral Vectors 101 blog posts Learn more about precise targeted manipulation Resources on Addgene.org ...

  6. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...datasets by computational biologists. Figure 1: The BioGRID Open Repository of CRISPR Screens (ORCS...resources on the Addgene blog Read this blog post about genome-wide screening using CRISPR Find all of our...on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for...

  7. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...proteins, DNA, and RNA used in CRISPR.   Figure 1: Overview of the parts of CRISPR. The bacterial chromosome...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral experience,...Nature and Science, for example). This blog post is about how to get the most out of your postdoctoral training...your new model?  PFs need to be regularly thinking about manuscripts. The other items will follow. If you...this is a strong indicator that this person cares about your science and your development. Taken together...Researchers face a fundamental conflict. Science is about exploration, discovering new information. There ...many PFs are reluctant to give talks or posters about their research. They dread giving a departmental...manuscript reviews, etc. You'll also meet future collaborators and get access to unpublished information and...

  9. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...target cell line or organism (Presented in figure 1). More information on Agrobacterium-mediated transformation...mediated cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation... Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits ...

  10. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...et al., 2019; McLaughlin, 2019).    Figure 1: Schematic of multiplex immunofluorescence approaches...). Springer New York. https://doi.org/10.1007/978-1-4939-8935-5_26 Jambor, H., Antonietti, A., Alicea,...to label the rat brain sample shown in Figure 2A above, Andrews et al. used the following combination of...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... antibody recognizes and binds to.   Figure 1: Labeled diagram of an antibody including Fc, Fab,...45. https://doi.org/10.1016/B978-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin ...is referred to as an antigen. You can learn more about antibodies in our Antibodies 101 animation, What... expressing this protein. Read on to learn more about antibodies and how to use them in the lab! Parts...lots is a big concern. You can find more details about polyclonal antibodies on our Antibodies 101: Polyclonal...not necessarily what you are getting. Learn more about monoclonal antibodies here. Recombinant antibodies... as well as compare between samples. Learn more about flow cytometry. Cell and tissue labeling Antibodies...

  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...you do just this, probably in less than a minute (1). Green Listed is a new software tool used to design...found through https://wermelinglab.com/.   References 1. Panda, Sudeepta Kumar, et al. "Green listed—a CRISPR... to design gRNAs for all the purposes discussed above. Please see videos and text on the website for more...Output: A text file containing full information about the suggested gRNAs to use, including all information... hard to read. It´s suggested that you open the above text files, copy the content of the file, and paste...Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for ...CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening Libraries Browse All...

  14. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging system...Avoid Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org...

  15. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...finally decide the LEGO laboratory was good enough. There are no pre-made LEGO laboratory sets so we had to ...everything now is about the plot. This part is easy, after all, who working in a laboratory has never faced...dysregulated water bath boiling your experiments, just about anything can disrupt your bench work and sometimes...to show people the very human struggles in the laboratory routine. We hope that sharing and laughing at...can learn with each other through globalized, collaborative thinking. LEGO and science: The potential is...while opening a LEGO set with my husband, I thought about how the limitless design possibilities of the LEGOs...

  16. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...share your science 1)   If you don’t already make it a habit to talk – really talk – about the research you...take on collaborative roles in academia, and that means it’s important to know how to talk about science...talking about and sharing some science on the one you already have. Read my earlier post about Scientists...important for scientists to get out there and talk about their work. To tell others why that work is cool... than ever before for scientists to communicate about their work and their interest in science more generally... the details of your science but also something about how science really works. 2)   Read science writing... told. Twitter can be a good way to follow news about particular subjects or find out what others are ...

  17. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...materials to the research community. References 1. Coroadinha, A. S., et al. "Effect of osmotic pressure...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

  18. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...in online patient/caregiver communities  Table 1: Examples of defining your audience for science communication...our Summer SciComm Series, where we’ll be talking about science communication, best practices in the field...research.   This summer, we’re making time to talk about my favorite thing - science communication (scicomm...endless.    Activity Example audience  Talking about your career as a scientist underrepresented minorities...students in AP biology classes Creating a podcast about genetics using dog breeds as an example adults ...get into the more exciting parts - learning more about science communication itself! See you next time!...

  19. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ... Figure 1: Antibody against PSD-95 from the Trimmer Lab. Information about the antibody, the... also be collecting feedback from the community about how the antibodies work in various applications,...antibodies are plasmid-based and recombinant. Learn more about recombinant antibodies here. Finally, Addgene is...blog and Antibody Guide, which include information about immunofluorescence, conjugates, isotypes, and more...learn more or get started.  If you have suggestions about how to make the resource better or ideas for new...

  20. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...class.   Four ways to improve your next manuscript 1. Be consistent with your figures The figures of a ...successful writer. When I originally talked to my PI about becoming a better writer, I was unsure if I should... a manuscript and critiquing it, were not too laborious, but helped build my understanding of how to generate...and the writing workshop. SWPI's writing workshop About a month later I received another email notifying... manuscript, scientists have a tendency to be elaborate and over explain the material. The point of writing...
Showing: 461 - 480 of 672 results