We narrowed to 709 results for: abo.1

...Protocols Publication describing them here. References 1.Ran, F. Ann, et al. "Genome engineering using the ...the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common...

...deletions, and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. 5. Five Popular...

...relations, advocacy, and writing legislation (see Figure 1 for career map). The beautiful part of science policy...policy as a profession. Figure 1: Science policy can lead you to many paths! For a more details,...learn more about their career trajectory. If there’s one thing everyone loves, it’s talking about themselves...learn more about the field.  Read the CCST Science Policy Career Guide to learn more about science policy...Administration, I could never get one straight answer about what science policy even is! In this article, I ... the #scipol hashtag. Often there will be posts about virtual meet ups and happy hours that you can attend...solving one problem. It often requires thinking about a problem in a new way through the integration of...

...guaranteed. To learn more about this process check out our Mouse Modeling blog posts: Part 1: Genetically Engineered...Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning... Delivery Methods Learn about Mouse Work on Exercise and Depression Read About Brainbow Resources on ...by CRISPR. The sequence started clean and then, about 100 bps into the run, it got “dirty”, with multiple...makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease...by Southern blot. From the efficiency discussed above extends 4 major advantages of CRISPR mouse editing...targeting. It also costs less. With CRISPR, it may cost about $5,000 to generate founder mice, while with conventional...

...furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse of...cellular analysis products at Promega.     References 1. Hall, M.P., et al. (2012) Engineered luciferase reporter...Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent Protein...heme-containing proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often... emits light at a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed...be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...

... and deep-brain fiber photometry.  Figure 1: Characterization of iGluSnFR4 in mice. A) Scheme ...CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-1 cells (colorectal cancer). Image courtesy of Brianna...insert your genetic cargo of choice. After hearing about researchers' favorite features and limitations of...development and cancer. But there is still much to learn about their myriad functions and therapeutic potential...created a very quick and efficient way to learn more about gene regulation. Find the TurboCas plasmid here!...Initiative Armamentarium Collection, a major collaborative effort to develop molecular tools for studying...

...strategies in the comments section!   References 1. Yearly Planning Meetings: Individualized Development... development at the center of her lab (pictured above). DePace holds yearly planning meetings with each... their work rather than overwhelmed or confused about what’s coming next. Goal setting and career planning...and their next steps. Addgene would love to hear about the steps taken in your lab to promote personal ...

...fingers of a completely normal size.      References: 1. Doench, John G., et al. "Optimized sgRNA design to...numbers that’ll leave you, well, #sad. Learn More About CRISPR Pooled Library Screening Why the kinome?...

...organized and boost your productivity in 2014. Tip 1: Use color coding, labels, and post-its to make finding...experiments before the next R01 is due. But what about organizing yourself and your lab? Organizing your...

...information Since the inception of Phage Directory ~1 year ago, Sacher and Zheng found that they could expand...open science Find blog posts about scientific sharing Find blog posts about networking Additional resources...

...and drawbacks of four split AAV vector approaches. 1. Overlapping  Overview: The overlapping strategy has...Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140 Frischmeyer...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...

...P2A-PuroR for lentiviral expression. Figure 1: dCas9-p300 adds H3K27ac marks to histones in promoter...lentiviral transduction. Lysine-specific Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited ...modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although...setting up your experimental system. Let us know about your experience with these constructs in the comments...

...reconstitutes a fully functional luciferase enzyme (Figure 1). Addition of substrate generates a proportionate ...questions before creating knock-ins.  Figure 1: Schematic of HiBiT tagging technology.   The...response-element NanoLuc reporter to show that the mutants abolished transcriptional activity, even in the presence...products offered by Promega. The vectors linked above are ready to request from Addgene so you can combine...

...to western blotting  In a western, proteins are: (1) separated by size, (2) transferred to a membrane,...  (3) detected using antibodies.   Figure 1: Overview of the Western blot process. The sample,... the thickness of the band provides information about the relative abundance of the protein in the sample...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...

... towards understanding this disease. Figure 1: Scientists are continuing to do essential research...blog Read about COVID-19 research on the blog Visit our guide for external collaborations Resources ...scientific sharing. Already we’ve seen articles about COVID-19 on preprint servers and in journals, and...vary, everyone we spoke with sees sharing and collaboration as the best way that the scientific community...useful resources during this time. Facilitate collaborations within the scientific community The team behind... Canada is a database that provides information about reagents, funding and volunteer opportunities, protocols...CCRP2) at Rutgers University not only supports collaborations at Rutgers, but also lists data and experimental...

... if the name was the correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  ... of Bioinformatics reached out to us to tell us about a resource called Cellosaurus, a growing cell line...all the information in the resource.  This is a labor-intensive project; scripts can be used in some cases...preferences to help you identify proximal lines you care about. If you have cell lines without a certified origin... Or finding errors in the available information about a cell line you’ve used? Submit your manuscript ...distributor. A success all around! If you’re excited about this resource and want to learn more, check out ...

...of experiments they are commonly used for.  Table 1. Points to Keep in Mind When Performing Biosensor ...sensors generally follow the same pattern described above - a sensing domain induces changes in a fluorescent...expressing a biosensor in eukaryotic cells, think about the best place for the biosensor to be expressed...MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor: which fluorescent...Blog Check out the Rosella Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome...

... email in a busy inbox. Updated policy As of July 1, 2025, we are updating our Hold for Publication policy... in the hold status and reach out to depositors about them, but we all know how easy it is to miss an ...happy to distribute unpublished materials. What about preprints? Addgene supports preprints and encourages...appreciate your feedback. If you have questions about depositing, check out our website, Help Center, ...

...literature? Very often the data of interest are in 1 or 2 figures from one paper and then another figure...ReFigure Analyzing replications as in this ReFigure about whether Natronobacterium gregoryi Argonaute has ...functionality Collecting preclinical and clinical data about a disease or therapeutic Side-by-side comparison...share your science at We Share Science Learn more about Data Sharing Resources at Addgene.org Deposit ...

...Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169. Soo, Rochelle M., et al... Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous...