We narrowed to 643 results for: ato

1. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   ...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

2. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   ...the antigen-binding sites of the antibody - the paratopes - may change and impact the specificity and avidity...

3. Communicating Your Science With Help From ComSciCon

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   Blog Post

   ...in all STEM fields become excellent science communicators. To empower graduate students to become ambassadors...

4. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...

5. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   ...been used in yeast by Lee et al (2013) and in nematodes by El Mouridi et al (2017) and Heppert et al (...

6. Multiplex Genome Editing with CRISPR-Cpf1

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   Blog Post

   ...multiplexing vectors are larger because they require regulatory sequences to allow for expression of multiple...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   Blog Post

   ...Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression...

8. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   Blog Post

   ...and leveraging tools like the Myers-Briggs Type Indicator or DISC assessments can help you navigate team...

9. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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   Blog Post

   ..., the stem-loop structure of transcriptional terminators. Another potential issue is sequence similarity...

10. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post

    ... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for...

11. Plasmids 101: Screens vs. Selections

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    Blog Post

    ...selected growth conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically...

12. Behind the scenes: Addgene’s new search engine and more

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    Blog Post

    ...of other websites, we built our new search engine atop Elasticsearch, a powerful open-source search engine...

13. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ..., Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible ...

14. How to Make Friends and Meet People at a Scientific Conference

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    Blog Post

    ...You never know when you will meet a potential collaborator Some of these people you meet will review your...

15. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post

    ...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ...

16. Bacterial Expression Systems

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    Collection

    ...Two-Hybrid System Various Plasmids placO with lambda-C1 operator Beta-galactosidase Ann Hochschild Bacterial two-hybrid...

17. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons, the channelrhodopsins...

18. RNA Extraction Without A Kit

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    Blog Post

    ...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...

19. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    Blog Post

    ...and were presented at the ASPB meeting by the collaborators who could participate. Despite the group not...

20. Summer SciComm: Preprints

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    Blog Post

    ..., social media, coffee chats with potential collaborators, blogging and more. These each have different...