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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 494 results for: control

Showing: 461 - 480 of 494 results

  6. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...samples being tested. Always include a No Template Control (NTC), i.e master mix + water Whenever possible...duplicate. Do not forget to include a no template control ( NTC = master mix + water ). Add 15 μL of Master...

  7. Kit Free RNA Extraction

    Type
    Protocol
    ...unlike using a p1000 tip it will give you more control of where you’re aiming your tip in the tube. Precipitate...

  10. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing non-hairpin insert. .... pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid... parallel. This plate will serve as a positive control for the puromycin selection. Days 4+: g. Change...

  11. Plan Your Experiment

    Type
    Guide
    ...CRISPR experiments and can be used as positive controls when working out a new protocol. Read more about...Weissman, J. S. (2014). Genome-Scale CRISPR-Mediated control of gene repression and activation. Cell , 159 (...

  15. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Engineering Using CRISPR/Cas Systems has expanded out of control. To further consolidate these FAQs, Le Cong worked...

  18. Centrifugation

    Type
    Protocol
    ...the rotor with microfuge tubes in place, and the control panel where you can set the time and speed needed...

  19. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...by researchers are used to detect, measure, and control molecular signals of cells (commonly neurons) in...release them. Optogenetics Biological technique to control the activity of cells, often neurons, with light...

  20. Ligation Independent Cloning

    Type
    Protocol
    ... your chances of success. Set up a vector only control with water instead of the insert. Incubate the ...
Showing: 461 - 480 of 494 results