We narrowed to 478 results for: mag

1. Plasmids 101: Using Transposons in the Lab

   Type

   Blog Post

   ...orientation. T-LR= transposons left-right orientation. Image from Leo Vo.   TL;DR Transposons are another...

2. Sequencing Options for CRISPR Genotyping

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   Blog Post

   ...equitable society. Read more at https://magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et...

3. 9 tips for a successful postdoctoral experience

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   Blog Post

   ...optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance ...

4. Pipetting Protocol

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   Protocol

   ...pipette with the appropriate dispense volume: This image shows the P2, P10, P20, P100, P200, and P1000 as...

5. Cancer and the Immune System: Deciphering the Relationship

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   Blog Post

   ...conventional therapies produce lasting side effects, damage healthy cells, and often cannot eradicate metastasis...

6. Antibodies 101: Flow Cytometry

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   Blog Post

   ... and permeabilization as these methods require damaging the cell membrane and will cause all cells to ...

7. Typing CRISPR Systems

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   Blog Post

   ...DNA after recruitment by Cascade. As you might imagine, this is handy for creating large genomic deletions...

8. With an Eye Towards the Future, We Look Back at the March for Science

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   Blog Post

   ...in a lab that uses yeast cells to study how DNA damage promotes cancer. She is also a science communicator...

9. Virus Protocol - Generating Stable Cell Lines

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   Protocol

   ...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

10. A Needle in a Base-Stack: Cas9 Structural Biology

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    Blog Post

    ...and doubles back on itself at the bottom of the image. Stem loops 1 and 2 extend horizontally across both...

11. Plasmid Cloning by PCR (with Protocols)

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    Protocol

    ...that will bind to and replicate it. The following image shows the ends of the ORF and how these are used...

12. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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    Blog Post

    ...transcriptase template; PBS = primer binding site. Image from Doman et al., 2023, under a CC BY 4.0 license...

13. Antibody Validation for Flow Cytometry

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    Blog Post

    ...the Attune NxT Flow Cytometer with representative images showing the staining intensity in the KO population...

14. Isolating a Monoclonal Cell Population by Limiting Dilution

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    Protocol

    ...Heat-inactivated FBS 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

15. AAV ddPCR Titration

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    Protocol

    ...loaded in wells A04, A05 and A06. As shown in the image (Figure 1) and table below, the concentration of...

16. Delivery Methods for Generating iPSCs

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    Blog Post

    ...at generating iPSCs, usually several orders of magnitude lower than retroviruses; however, because they...

17. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post

    ...nondividing cells. Scientists everywhere wistfully imagined being able to specify and correct single-base ...

18. Protocol - pLKO.1 – TRC Cloning Vector

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    Protocol

    ...wavelength UV light will increase the chance of damaging the DNA. Purify the DNA using a Qiaquick gel extraction...