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1. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

2. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ...that will bind to and replicate it. The following image shows the ends of the ORF and how these are used...

3. A Needle in a Base-Stack: Cas9 Structural Biology

   Type

   Blog Post

   ...and doubles back on itself at the bottom of the image. Stem loops 1 and 2 extend horizontally across both...

4. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

   Type

   Blog Post

   ...transcriptase template; PBS = primer binding site. Image from Doman et al., 2023, under a CC BY 4.0 license...

5. Antibody Validation for Flow Cytometry

   Type

   Blog Post

   ...the Attune NxT Flow Cytometer with representative images showing the staining intensity in the KO population...

6. Isolating a Monoclonal Cell Population by Limiting Dilution

   Type

   Protocol

   ...Heat-inactivated FBS 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

7. AAV ddPCR Titration

   Type

   Protocol

   ...loaded in wells A04, A05 and A06. As shown in the image (Figure 1) and table below, the concentration of...

8. Delivery Methods for Generating iPSCs

   Type

   Blog Post

   ...at generating iPSCs, usually several orders of magnitude lower than retroviruses; however, because they...

9. CRISPR 101: Cytosine and Adenine Base Editors

   Type

   Blog Post

   ...nondividing cells. Scientists everywhere wistfully imagined being able to specify and correct single-base ...

10. Protocol - pLKO.1 – TRC Cloning Vector

    Type

    Protocol

    ...wavelength UV light will increase the chance of damaging the DNA. Purify the DNA using a Qiaquick gel extraction...